Dipyridamole inhibits in vitro renal fibroblast proliferation and collagen synthesis

• Published in: The Journal of laboratory and clinical medicine 2002-09, Vol.140 (3), p.199-208
• Main Authors: Hewitson, Tim D., Tait, Melanie G., Kelynack, Kristen J., Martic, Marina, Becker, Gavin J.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cell Division; Cell Survival - drug effects; Cells, Cultured; Collagen - biosynthesis; Collagen - genetics; Dipyridamole - pharmacology; Disease Models, Animal; Fibroblasts - drug effects; Fibroblasts - metabolism; Fibroblasts - pathology; Fluorescent Antibody Technique, Indirect; Immunoenzyme Techniques; In Situ Hybridization; Indoles - pharmacology; Isoquinolines - pharmacology; Kidney - drug effects; Kidney - metabolism; Kidney - pathology; Male; Maleimides - pharmacology; Medical sciences; Nitric Oxide - metabolism; Pharmacology. Drug treatments; Phosphodiesterase Inhibitors - pharmacology; Rats; Rats, Sprague-Dawley; RNA, Messenger - metabolism; Sulfonamides; Ureteral Obstruction; Urinary system
• ISSN: 0022-2143; 1532-6543
• DOI: 10.1067/mlc.2002.126828

Fibroblasts are universally recognized in situations of tubulointerstitial injury, where their presence has been shown to be a marker of disease progression. The objective of this study was to determine whether the functions of fibroblasts relevant to fibrogenesis can be modified in vitro with dipyridamole. Cells were obtained from obstructed rat renal tissue and characterized on the basis of immunohistochemical findings. Fibroblasts constituted all of the cells from passage 3. Functional parameters were measured in cells cultured with 1, 5, and 50 μmol/L dipyridamole and compared to basal parameters of cells grown in Dulbecco's modified Eagle's medium plus 10% fetal calf serum (control). Northern-blot analysis indicated that dipyridamole decreased procollagen α1(I) messenger ribonucleic acid expression (P