Apolipoprotein D transcription occurs specifically in nonproliferating quiescent and senescent fibroblast cultures

We studied apolipoprotein D (apoD) mRNA in primary cultures of human diploid fibroblasts (HDF). In early-passage HDF no apoD mRNA was detected in replicating cells in sparse culture, but the gene was expressed in quiescent cells in confluent and in serum-starved cultures. In contrast, late-passage H...

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Bibliographic Details
Published in:FEBS letters 1991-09, Vol.290 (1), p.139-141
Main Authors: Provost, Pierre R., Marcel, Yves L., Milne, Ross W., Weech, Philip K., Rassart, Eric
Format: Article
Language:English
Subjects:
Apolipoproteins - genetics
Apolipoproteins D
Biological and medical sciences
Blotting, Northern
Cell Division
Cell Line
Cellular Senescence
Fibroblasts - cytology
Fibroblasts - physiology
Fundamental and applied biological sciences. Psychology
GCDFP
Gene Expression
gross-cystic-disease-fluid protein
Growth arrest
HDF
human diploid fibroblast
Humans
In Vitro Techniques
Lipocalin (α2u-globulin) family
Molecular and cellular biology
Molecular genetics
PDLs
population doubling levels
RNA, Messenger - genetics
Transcription, Genetic
Transcription. Transcription factor. Splicing. Rna processing
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Summary:We studied apolipoprotein D (apoD) mRNA in primary cultures of human diploid fibroblasts (HDF). In early-passage HDF no apoD mRNA was detected in replicating cells in sparse culture, but the gene was expressed in quiescent cells in confluent and in serum-starved cultures. In contrast, late-passage HDF expressed apoD mRNA in sparse culture, but the level increased after attainment of confluence. Thus fibroblasts, the common cell-type expressing apoD mRNA in vivo, express this characteristic following growth-arrest. The same pattern of activation was found in another fibroblast cell line deficient in apoB/E (LDL) receptors, excluding a role for cellular cholesterol delivery by the LDL-receptor pathway controlling apoD expression
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(91)81244-3