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Apolipoprotein D transcription occurs specifically in nonproliferating quiescent and senescent fibroblast cultures

We studied apolipoprotein D (apoD) mRNA in primary cultures of human diploid fibroblasts (HDF). In early-passage HDF no apoD mRNA was detected in replicating cells in sparse culture, but the gene was expressed in quiescent cells in confluent and in serum-starved cultures. In contrast, late-passage H...

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Published in:	FEBS letters 1991-09, Vol.290 (1), p.139-141
Main Authors:	Provost, Pierre R., Marcel, Yves L., Milne, Ross W., Weech, Philip K., Rassart, Eric
Format:	Article
Language:	English
Subjects:	Apolipoproteins - genetics Apolipoproteins D Biological and medical sciences Blotting, Northern Cell Division Cell Line Cellular Senescence Fibroblasts - cytology Fibroblasts - physiology Fundamental and applied biological sciences. Psychology GCDFP Gene Expression gross-cystic-disease-fluid protein Growth arrest HDF human diploid fibroblast Humans In Vitro Techniques Lipocalin (α2u-globulin) family Molecular and cellular biology Molecular genetics PDLs population doubling levels RNA, Messenger - genetics Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing
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creator	Provost, Pierre R. Marcel, Yves L. Milne, Ross W. Weech, Philip K. Rassart, Eric
description	We studied apolipoprotein D (apoD) mRNA in primary cultures of human diploid fibroblasts (HDF). In early-passage HDF no apoD mRNA was detected in replicating cells in sparse culture, but the gene was expressed in quiescent cells in confluent and in serum-starved cultures. In contrast, late-passage HDF expressed apoD mRNA in sparse culture, but the level increased after attainment of confluence. Thus fibroblasts, the common cell-type expressing apoD mRNA in vivo, express this characteristic following growth-arrest. The same pattern of activation was found in another fibroblast cell line deficient in apoB/E (LDL) receptors, excluding a role for cellular cholesterol delivery by the LDL-receptor pathway controlling apoD expression
doi_str_mv	10.1016/0014-5793(91)81244-3
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The same pattern of activation was found in another fibroblast cell line deficient in apoB/E (LDL) receptors, excluding a role for cellular cholesterol delivery by the LDL-receptor pathway controlling apoD expression</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(91)81244-3</identifier><identifier>PMID: 1915865</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Apolipoproteins - genetics ; Apolipoproteins D ; Biological and medical sciences ; Blotting, Northern ; Cell Division ; Cell Line ; Cellular Senescence ; Fibroblasts - cytology ; Fibroblasts - physiology ; Fundamental and applied biological sciences. Psychology ; GCDFP ; Gene Expression ; gross-cystic-disease-fluid protein ; Growth arrest ; HDF ; human diploid fibroblast ; Humans ; In Vitro Techniques ; Lipocalin (α2u-globulin) family ; Molecular and cellular biology ; Molecular genetics ; PDLs ; population doubling levels ; RNA, Messenger - genetics ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. 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In early-passage HDF no apoD mRNA was detected in replicating cells in sparse culture, but the gene was expressed in quiescent cells in confluent and in serum-starved cultures. In contrast, late-passage HDF expressed apoD mRNA in sparse culture, but the level increased after attainment of confluence. Thus fibroblasts, the common cell-type expressing apoD mRNA in vivo, express this characteristic following growth-arrest. The same pattern of activation was found in another fibroblast cell line deficient in apoB/E (LDL) receptors, excluding a role for cellular cholesterol delivery by the LDL-receptor pathway controlling apoD expression</description><subject>Apolipoproteins - genetics</subject><subject>Apolipoproteins D</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Cell Division</subject><subject>Cell Line</subject><subject>Cellular Senescence</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GCDFP</subject><subject>Gene Expression</subject><subject>gross-cystic-disease-fluid protein</subject><subject>Growth arrest</subject><subject>HDF</subject><subject>human diploid fibroblast</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Lipocalin (α2u-globulin) family</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>PDLs</subject><subject>population doubling levels</subject><subject>RNA, Messenger - genetics</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. 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Psychology</topic><topic>GCDFP</topic><topic>Gene Expression</topic><topic>gross-cystic-disease-fluid protein</topic><topic>Growth arrest</topic><topic>HDF</topic><topic>human diploid fibroblast</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Lipocalin (α2u-globulin) family</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>PDLs</topic><topic>population doubling levels</topic><topic>RNA, Messenger - genetics</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. 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subjects	Apolipoproteins - genetics Apolipoproteins D Biological and medical sciences Blotting, Northern Cell Division Cell Line Cellular Senescence Fibroblasts - cytology Fibroblasts - physiology Fundamental and applied biological sciences. Psychology GCDFP Gene Expression gross-cystic-disease-fluid protein Growth arrest HDF human diploid fibroblast Humans In Vitro Techniques Lipocalin (α2u-globulin) family Molecular and cellular biology Molecular genetics PDLs population doubling levels RNA, Messenger - genetics Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing
title	Apolipoprotein D transcription occurs specifically in nonproliferating quiescent and senescent fibroblast cultures
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