Structure of the O-specific polysaccharide of Proteus penneri 103 containing ribitol and 2-aminoethanol phosphates

The O-specific polysaccharide of the lipopolysaccharide of Proteus penneri strain 103 was studied using 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, H-detected 1H, 13C HMQC, 1H, 31P HMQC, and HMBC experiments. It was found that the polysaccharide is built up of oligosaccharide-ribit...

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Bibliographic Details
Published in:Carbohydrate research 2002-09, Vol.337 (17), p.1535-1540
Main Authors: Drzewiecka, Dominika, Toukach, Philip V, Arbatsky, Nikolay P, Zych, Krystyna, Shashkov, Alexander S, Knirel, Yuriy A, Sidorczyk, Zygmunt
Format: Article
Language:English
Subjects:
1H, 13C, 31P NMR
Carbohydrate Sequence
Ethanolamine - chemistry
Lipopolysaccharide
Magnetic Resonance Spectroscopy
Molecular Sequence Data
O Antigens - chemistry
O-antigen
O-specific polysaccharide
Phosphates - chemistry
Proteus - chemistry
Proteus penneri
Ribitol - chemistry
Ribitol phosphate, Ethanolamine phosphate
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Summary:The O-specific polysaccharide of the lipopolysaccharide of Proteus penneri strain 103 was studied using 1H and 13C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, H-detected 1H, 13C HMQC, 1H, 31P HMQC, and HMBC experiments. It was found that the polysaccharide is built up of oligosaccharide-ribitol phosphate repeating units and thus resembles ribitol teichoic acids of Gram-positive bacteria. The following structure of the polysaccharide was established: where Etn and Rib-ol are ethanolamine and ribitol, respectively. This structure is unique among the known structures of Proteus O-antigens and, therefore, we propose classification of the strain studied into a new Proteus serogroup, O73. The molecular basis for cross-reactivity between O-antiserum against P. penneri 103 and O-antigens of P. mirabilis O33 and D52 is discussed. Graphic
ISSN:0008-6215
1873-426X
DOI:10.1016/S0008-6215(02)00265-3