Synaptic relationships between hair follicle afferents and neurones expressing GABA and glycine-like immunoreactivity in the spinal cord of the rat

• Published in: Journal of comparative neurology (1911) 2002-10, Vol.452 (4), p.367-380
• Main Authors: Watson, Alan H. D., Hughes, David I., Bazzaz, Ayoub A.
• Format: Article
• Language: English
• Subjects: Animals; Anterior Horn Cells - chemistry; Anterior Horn Cells - physiology; Anterior Horn Cells - ultrastructure; Axons - chemistry; Axons - ultrastructure; Dendrites - chemistry; Dendrites - ultrastructure; dorsal horn; gamma-Aminobutyric Acid - analysis; gamma-Aminobutyric Acid - immunology; Glycine - analysis; Glycine - immunology; Hair Follicle - innervation; Immunohistochemistry; Microscopy, Immunoelectron; Neural Inhibition - physiology; Posterior Horn Cells - chemistry; Posterior Horn Cells - physiology; Posterior Horn Cells - ultrastructure; presynaptic inhibition; primary afferent depolarization; Rats; Rats, Inbred Strains - physiology; sensory neurone; Synapses - chemistry; Synapses - physiology; Synapses - ultrastructure; ventral horn
• ISSN: 0021-9967; 1096-9861
• DOI: 10.1002/cne.10410

γ‐Aminobutyric acid (GABA) and glycine have been implicated in the inhibition of sensory pathways in the dorsal horn of the spinal cord. The object of this study is to investigate the interactions between neurones immunoreactive for GABA and/or glycine and hair follicle afferent terminals labelled by intracellular injection with neurobiotin. GABA and glycine‐like immunoreactivity in axons and dendrites in synaptic contact with the afferent terminals was demonstrated by using a postembedding immunogold method, and serial section reconstruction was used to show the distribution and nature of these interactions in lamina III of the dorsal horn. Most afferent boutons (94%) were postsynaptic at axo‐axonic synapses: 67% of presynaptic boutons presynaptic to the afferent terminals were immunoreactive for GABA and glycine, 24% for GABA alone, and 7% for glycine alone. Only a small percentage of dendrites postsynaptic to afferent boutons appeared to belong to inhibitory interneurones: 3% were immunoreactive for GABA and glycine, 10% for glycine alone, but 87% were immunoreactive for neither antibody. Many afferent boutons were the central terminals of what appeared to be type IIb glomeruli and were involved triadic synaptic arrangements at which boutons presynaptic to an afferent terminal also made axodendritic contacts with dendrites postsynaptic to the afferent. Many of the presynaptic boutons involved in the triads were immunoreactive for GABA and glycine. Because afferent terminals do not themselves express glycine receptors (Mitchell et al. [1993] J. Neurosci. 13:2371–2381), glycine may therefore act on dendrites postsynaptic to hair follicle afferent terminals at these triads. J. Comp. Neurol. 452:367–380, 2002. © 2002 Wiley‐Liss, Inc.