The liver and the hematolymphoid system: I. The regulation of nylon-passed spleen cell proliferation by active factors released from syngeneic nonparenchymal liver cells

• Published in: Journal of leukocyte biology 1991-10, Vol.50 (4), p.402-411
• Main Authors: Mabuchi, Ayako, Komuro, Toshiro, Saizawa, Takashi, Sakamoto, Toshiki, Watari, Eiji, Yokomuro, Kozo
• Format: Article
• Language: English
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Animals; Biological and medical sciences; Cell Division - physiology; Cells, Cultured; colony‐stimulating factor; Culture Media - analysis; Culture Media - pharmacology; DNA - metabolism; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Granulocyte-Macrophage Colony-Stimulating Factor - analysis; Granulocyte-Macrophage Colony-Stimulating Factor - metabolism; Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology; Immune Sera; Immunobiology; interferon; Interferons - analysis; Interferons - metabolism; Interferons - pharmacology; interleukin; Interleukin-1 - analysis; Interleukin-1 - metabolism; Interleukin-1 - pharmacology; Interleukin-2 - metabolism; Interleukin-3 - metabolism; Interleukin-6 - analysis; Interleukin-6 - metabolism; Interleukin-6 - pharmacology; Kupffer cell; Liver - cytology; Liver - metabolism; Liver - physiology; Mice; Mice, Inbred C3H; Miscellaneous; Regulatory factors and their cellular receptors; Spleen - cytology; Spleen - metabolism; Spleen - physiology; Time Factors
• ISSN: 0741-5400; 1938-3673
• DOI: 10.1002/jlb.50.4.402

Nylon‐passed spleen cells were found to proliferate when cultured with syngeneic nonparenchymal adherent liver cells and their culture supernatants. The supernatants contained IL‐1, IL‐6, GM‐CSF, and IFN (α + β) activities but not IL‐2 and IL‐3 activities. The IFN level was higher in early culture sup (2–24 hr) than in later culture sup (48–72 hr). Proliferation was greatly increased by anti‐IFN (α + β) serum in the spleen cells cultured in the earlier sup. This antiserum increased the spleen cell proliferation only slightly in the later culture sup. This suggests that nonparenchymal liver cells produce two factors, one having a suppressor, and the other an enhancer action, with IFN being one of the suppressor factors. With culture time, DNA synthesis of spleen cells increased and IL‐2 and IL‐3 activities were generated in the culture sup. Cells proliferated during culture were found to be morphologically lymphocytes, granulocytes, and macrophages. The mechanisms by which nonparenchymal liver cells regulate the hematolymphoid system are discussed based on our observations.