Fluorimetric determination of some thiol compounds in their dosage forms

A simple fluorimetric procedure was adopted for determination of three pharmaceutical compounds containing thiol groups namely, captopril, d-penicillamine and N-acetylcysteine. In this method, the drugs are treated with 1,2-naphthoquinone-4-sulfonic acid. The latter is reduced to 1,2-dihydroxynaphth...

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Bibliographic Details
Published in:Farmaco (Società chimica italiana : 1989) 2002-08, Vol.57 (8), p.625-629
Main Authors: Al-Ghannam, Sh.M, El-Brashy, A.M, Al-Farhan, B.S
Format: Article
Language:English
Subjects:
1,2-Naphthoquinone-4-sulfonic acid
Analysis
Biological and medical sciences
Captopril
d-penicillamine
Dosage Forms
General pharmacology
Medical sciences
N-acetylcysteine
Pharmacology. Drug treatments
Spectrometry, Fluorescence - methods
Sulfhydryl Compounds - analysis
Sulfhydryl Compounds - chemistry
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Summary:A simple fluorimetric procedure was adopted for determination of three pharmaceutical compounds containing thiol groups namely, captopril, d-penicillamine and N-acetylcysteine. In this method, the drugs are treated with 1,2-naphthoquinone-4-sulfonic acid. The latter is reduced to 1,2-dihydroxynaphthalene-4-sulfonic acid which has a maximum fluorescence intensity at 480/318 nm ( λEm/Ex). The method is sensitive to 0.5–4.5 μg ml −1 with minimum detectability 0.05 μg ml −1 ( S/ N=2), and has been applied to determine these three thiols in their dosage forms. The results obtained are compared favourably with those obtained by their pharmacopeial methods.
ISSN:0014-827X
1879-0569
DOI:10.1016/S0014-827X(02)01223-5