Fluoroquinolone resistance in Mycoplasma gallisepticum: DNA gyrase as primary target of enrofloxacin and impact of mutations in topoisomerases on resistance level

Resistant mutants of Mycoplasma gallisepticum were selected in vitro by passaging strains 10 times in increasing concentrations of enrofloxacin. The regions of gyrA/gyrB and parC/parE, encoding the quinolone resistance-determining regions (QRDRs) of DNA gyrase and DNA topoisomerase IV, respectively,...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2002-10, Vol.50 (4), p.589-592
Main Authors: Reinhardt, A. K., Kempf, I., Kobisch, M., Gautier-Bouchardon, A. V.
Format: Article
Language:English
Subjects:
Anti-Infective Agents - pharmacology
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
DNA Gyrase - metabolism
DNA Topoisomerases - genetics
Drug Resistance, Bacterial - genetics
Enrofloxacin
Fluoroquinolones
Medical sciences
Mutation - genetics
Mycoplasma - drug effects
Mycoplasma - genetics
Pharmacology. Drug treatments
Quinolones - pharmacology
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Summary:Resistant mutants of Mycoplasma gallisepticum were selected in vitro by passaging strains 10 times in increasing concentrations of enrofloxacin. The regions of gyrA/gyrB and parC/parE, encoding the quinolone resistance-determining regions (QRDRs) of DNA gyrase and DNA topoisomerase IV, respectively, of the mutants obtained during different passages were sequenced. Several mutations were found in the four fluoroquinolone targets. Substitution of Ser-83→Arg in GyrA and Ser-80→Leu or Trp in ParC QRDRs seem to have the greatest impact on resistance to fluoroquinolones. The results obtained also suggest that the preferential target of enrofloxacin in M. gallisepticum is DNA gyrase.
ISSN:0305-7453
1460-2091
1460-2091
DOI:10.1093/jac/dkf158