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Role of the gag and pol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: an ultrastructural study

1 Department of Pathology and 2 AIDS Research Center, National Institute of Health, 2-10-35, Kamiosaki, Shinagawa-ku, Tokyo 141 3 Biological Science Laboratory, Nippon Zeon Company Ltd, Yako, Kawasaki-ku, Kawasaki-shi, Kanagawa, and 4 Basic Research Laboratories, Toray Industries Inc., Tebiro, Kamak...

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Published in:Journal of general virology 1991-10, Vol.72 (10), p.2509-2517
Main Authors: Hoshikawa, Nariyoshi, Kojima, Asato, Yasuda, Atsushi, Takayashiki, Eiko, Masuko, Sanae, Chiba, Joe, Sata, Tetsutaro, Kurata, Takeshi
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container_end_page 2517
container_issue 10
container_start_page 2509
container_title Journal of general virology
container_volume 72
creator Hoshikawa, Nariyoshi
Kojima, Asato
Yasuda, Atsushi
Takayashiki, Eiko
Masuko, Sanae
Chiba, Joe
Sata, Tetsutaro
Kurata, Takeshi
description 1 Department of Pathology and 2 AIDS Research Center, National Institute of Health, 2-10-35, Kamiosaki, Shinagawa-ku, Tokyo 141 3 Biological Science Laboratory, Nippon Zeon Company Ltd, Yako, Kawasaki-ku, Kawasaki-shi, Kanagawa, and 4 Basic Research Laboratories, Toray Industries Inc., Tebiro, Kamakura, Kanagawa, Japan An ultrastructural study was performed on rabbit epithelial RK-13 cells and CD4 + human T lymphocyte lines infected with various recombinant vaccinia viruses (RVVs) expressing genes of human immunodeficiency virus (HIV): the mature p17 or p24 gag domain alone, the entire or truncated gag gene, the reverse transcriptase domain, or the gag-pol genes with a frameshift mutation. Cells infected with RVVs that produced the gag polyprotein with a predicted M r of more than 48K showed budding and release of HIV-like particles into the extracellular space. These particles were not observed in cells expressing a truncated gag gene (p17 and p24 regions). Mature HIV-like particles were observed extracellularly when the entire gag gene and the protease region of the pol gene were expressed. In contrast, in cells infected with RVVs that contained the gag-pol gene with a frameshift mutation, neither recognizable budding structures nor extracellular HIV-like particles could be detected. These results suggest that the gag gene, particularly its 3' terminus, is necessary for the assembly of HIV particles. In addition, the protease region of the pol gene seems to be required for morphological maturation of HIV particles, but complete proteolytic cleavage of the gag protein may prevent bud formation. Received 4 March 1991; accepted 19 June 1991.
doi_str_mv 10.1099/0022-1317-72-10-2509
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ispartof Journal of general virology, 1991-10, Vol.72 (10), p.2509-2517
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1465-2099
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source Freely Accessible Science Journals - check A-Z of ejournals
subjects AIDS/HIV
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Blotting, Western
Cell Line
Cloning, Molecular
DNA, Viral
Fundamental and applied biological sciences. Psychology
Genes, gag
Genes, pol
Genetics
HIV-1 - genetics
HIV-1 - growth & development
HIV-1 - ultrastructure
Humans
Microbiology
Molecular Sequence Data
Morphogenesis
Rabbits
vaccinia virus
Vaccinia virus - genetics
Virion - genetics
Virion - metabolism
Virology
title Role of the gag and pol genes of human immunodeficiency virus in the morphogenesis and maturation of retrovirus-like particles expressed by recombinant vaccinia virus: an ultrastructural study
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