In vitro isolation of equine piroplasms derived from Cape Mountain zebra (Equus zebra zebra) in South Africa

Twenty blood samples of zebras (Equus zebra zebra) from the Karoo National Park and the Bontebok National Park in South Africa, all seropositive for Theileria equi, were subjected to in vitro culture to identify carrier animals and to isolate the parasites. Sixteen animals had a detectable parasitae...

Full description

Saved in:
Bibliographic Details
Published in:Onderstepoort journal of veterinary research 2002-09, Vol.69 (3), p.197-200
Main Authors: Zweygarth, E, Lopez-Rebollar, L.M. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.), Meyer, P
Format: Article
Language:English
Subjects:
AFRIQUE DU SUD
Animals
Babesia - isolation & purification
BABESIA CABALLI
BABESIA EQUI
Babesiosis - diagnosis
Babesiosis - epidemiology
Babesiosis - veterinary
Carrier State - diagnosis
Carrier State - veterinary
CULTIVO IN VITRO
CULTURE IN VITRO
Equidae - parasitology
IN VITRO CULTURE
In Vitro Techniques
Parasitemia - diagnosis
Parasitemia - epidemiology
Parasitemia - veterinary
PIROPLASMEA
Seroepidemiologic Studies
SOUTH AFRICA
SUDAFRICA
THEILERIA
Theileria - isolation & purification
Theileriasis - diagnosis
Theileriasis - epidemiology
ZEBRA
ZEBRAS
ZEBRE
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Twenty blood samples of zebras (Equus zebra zebra) from the Karoo National Park and the Bontebok National Park in South Africa, all seropositive for Theileria equi, were subjected to in vitro culture to identify carrier animals and to isolate the parasites. Sixteen animals had a detectable parasitaemia in Giemsa-stained blood smears examined before culture initiation, the remaining four animals were identified as T. equi carriers by in vitro culture. Cultures were initiated either in an oxygen-reduced gas mixture or in a 5% CO2-in-air atmosphere. Out of the 20 blood samples, 12 cultures of T. equi and two cultures of T. equi mixed with Babesia caballi were established. None of the four animals seropositive for B. caballi could be identified as carrier animals, whereas two seronegative samples became culture-positive for B. caballi.
ISSN:0030-2465
2219-0635