Loading…
In vitro isolation of equine piroplasms derived from Cape Mountain zebra (Equus zebra zebra) in South Africa
Twenty blood samples of zebras (Equus zebra zebra) from the Karoo National Park and the Bontebok National Park in South Africa, all seropositive for Theileria equi, were subjected to in vitro culture to identify carrier animals and to isolate the parasites. Sixteen animals had a detectable parasitae...
Saved in:
| Published in: | Onderstepoort journal of veterinary research 2002-09, Vol.69 (3), p.197-200 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | |
|---|---|
| cites | |
| container_end_page | 200 |
| container_issue | 3 |
| container_start_page | 197 |
| container_title | Onderstepoort journal of veterinary research |
| container_volume | 69 |
| creator | Zweygarth, E Lopez-Rebollar, L.M. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.) Meyer, P |
| description | Twenty blood samples of zebras (Equus zebra zebra) from the Karoo National Park and the Bontebok National Park in South Africa, all seropositive for Theileria equi, were subjected to in vitro culture to identify carrier animals and to isolate the parasites. Sixteen animals had a detectable parasitaemia in Giemsa-stained blood smears examined before culture initiation, the remaining four animals were identified as T. equi carriers by in vitro culture. Cultures were initiated either in an oxygen-reduced gas mixture or in a 5% CO2-in-air atmosphere. Out of the 20 blood samples, 12 cultures of T. equi and two cultures of T. equi mixed with Babesia caballi were established. None of the four animals seropositive for B. caballi could be identified as carrier animals, whereas two seronegative samples became culture-positive for B. caballi. |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_72144215</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>246376681</sourcerecordid><originalsourceid>FETCH-LOGICAL-f256t-77f5cda4fc7b3098f520737c2783459f67391beeb308a4cb701b7b642dd6149e3</originalsourceid><addsrcrecordid>eNpdkE9LxDAQxYMo7rr6EZTgQfRQyP80x2VZdUHxoF68lLRNNEvbdJNmQT-91V0vwvCG4f2YN8wBmBKCVYYE5YdgihBFGWGCT8BJjGuEsCIKHYMJJpQLLPgUNKsObt0QPHTRN3pwvoPeQrNJrjOwd8H3jY5thLUJbmtqaINv4UL3Bj761A3adfDLlEHD6-UmpbgffvUGjuazT8MHnNvgKn0Kjqxuojnb9xl4vV2-LO6zh6e71WL-kFnCxZBJaXlVa2YrWVKkcssJklRWROaUcWWFpAqXxoxmrllVSoRLWQpG6lpgpgydgavd3j74TTJxKFoXK9M0ujM-xUISzBjBfAQv_4Frn0I33lZgxVE-BvxAF3sola2piz64VofP4u-JI3C-A6z2hX4PLhZvc4LQWEgJTL8B0ut2ng</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>195083085</pqid></control><display><type>article</type><title>In vitro isolation of equine piroplasms derived from Cape Mountain zebra (Equus zebra zebra) in South Africa</title><source>Publicly Available Content Database</source><creator>Zweygarth, E ; Lopez-Rebollar, L.M. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.) ; Meyer, P</creator><creatorcontrib>Zweygarth, E ; Lopez-Rebollar, L.M. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.) ; Meyer, P</creatorcontrib><description>Twenty blood samples of zebras (Equus zebra zebra) from the Karoo National Park and the Bontebok National Park in South Africa, all seropositive for Theileria equi, were subjected to in vitro culture to identify carrier animals and to isolate the parasites. Sixteen animals had a detectable parasitaemia in Giemsa-stained blood smears examined before culture initiation, the remaining four animals were identified as T. equi carriers by in vitro culture. Cultures were initiated either in an oxygen-reduced gas mixture or in a 5% CO2-in-air atmosphere. Out of the 20 blood samples, 12 cultures of T. equi and two cultures of T. equi mixed with Babesia caballi were established. None of the four animals seropositive for B. caballi could be identified as carrier animals, whereas two seronegative samples became culture-positive for B. caballi.</description><identifier>ISSN: 0030-2465</identifier><identifier>EISSN: 2219-0635</identifier><identifier>PMID: 12356165</identifier><identifier>CODEN: OJVRAZ</identifier><language>eng</language><publisher>South Africa: AOSIS (Pty) Ltd</publisher><subject>AFRIQUE DU SUD ; Animals ; Babesia - isolation & purification ; BABESIA CABALLI ; BABESIA EQUI ; Babesiosis - diagnosis ; Babesiosis - epidemiology ; Babesiosis - veterinary ; Carrier State - diagnosis ; Carrier State - veterinary ; CULTIVO IN VITRO ; CULTURE IN VITRO ; Equidae - parasitology ; IN VITRO CULTURE ; In Vitro Techniques ; Parasitemia - diagnosis ; Parasitemia - epidemiology ; Parasitemia - veterinary ; PIROPLASMEA ; Seroepidemiologic Studies ; SOUTH AFRICA ; SUDAFRICA ; THEILERIA ; Theileria - isolation & purification ; Theileriasis - diagnosis ; Theileriasis - epidemiology ; ZEBRA ; ZEBRAS ; ZEBRE</subject><ispartof>Onderstepoort journal of veterinary research, 2002-09, Vol.69 (3), p.197-200</ispartof><rights>Copyright Onderstepoort Veterinary Institute Sep 2002</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/195083085/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/195083085?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,37012,37013,44590,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12356165$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zweygarth, E</creatorcontrib><creatorcontrib>Lopez-Rebollar, L.M. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.)</creatorcontrib><creatorcontrib>Meyer, P</creatorcontrib><title>In vitro isolation of equine piroplasms derived from Cape Mountain zebra (Equus zebra zebra) in South Africa</title><title>Onderstepoort journal of veterinary research</title><addtitle>Onderstepoort J Vet Res</addtitle><description>Twenty blood samples of zebras (Equus zebra zebra) from the Karoo National Park and the Bontebok National Park in South Africa, all seropositive for Theileria equi, were subjected to in vitro culture to identify carrier animals and to isolate the parasites. Sixteen animals had a detectable parasitaemia in Giemsa-stained blood smears examined before culture initiation, the remaining four animals were identified as T. equi carriers by in vitro culture. Cultures were initiated either in an oxygen-reduced gas mixture or in a 5% CO2-in-air atmosphere. Out of the 20 blood samples, 12 cultures of T. equi and two cultures of T. equi mixed with Babesia caballi were established. None of the four animals seropositive for B. caballi could be identified as carrier animals, whereas two seronegative samples became culture-positive for B. caballi.</description><subject>AFRIQUE DU SUD</subject><subject>Animals</subject><subject>Babesia - isolation & purification</subject><subject>BABESIA CABALLI</subject><subject>BABESIA EQUI</subject><subject>Babesiosis - diagnosis</subject><subject>Babesiosis - epidemiology</subject><subject>Babesiosis - veterinary</subject><subject>Carrier State - diagnosis</subject><subject>Carrier State - veterinary</subject><subject>CULTIVO IN VITRO</subject><subject>CULTURE IN VITRO</subject><subject>Equidae - parasitology</subject><subject>IN VITRO CULTURE</subject><subject>In Vitro Techniques</subject><subject>Parasitemia - diagnosis</subject><subject>Parasitemia - epidemiology</subject><subject>Parasitemia - veterinary</subject><subject>PIROPLASMEA</subject><subject>Seroepidemiologic Studies</subject><subject>SOUTH AFRICA</subject><subject>SUDAFRICA</subject><subject>THEILERIA</subject><subject>Theileria - isolation & purification</subject><subject>Theileriasis - diagnosis</subject><subject>Theileriasis - epidemiology</subject><subject>ZEBRA</subject><subject>ZEBRAS</subject><subject>ZEBRE</subject><issn>0030-2465</issn><issn>2219-0635</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNpdkE9LxDAQxYMo7rr6EZTgQfRQyP80x2VZdUHxoF68lLRNNEvbdJNmQT-91V0vwvCG4f2YN8wBmBKCVYYE5YdgihBFGWGCT8BJjGuEsCIKHYMJJpQLLPgUNKsObt0QPHTRN3pwvoPeQrNJrjOwd8H3jY5thLUJbmtqaINv4UL3Bj761A3adfDLlEHD6-UmpbgffvUGjuazT8MHnNvgKn0Kjqxuojnb9xl4vV2-LO6zh6e71WL-kFnCxZBJaXlVa2YrWVKkcssJklRWROaUcWWFpAqXxoxmrllVSoRLWQpG6lpgpgydgavd3j74TTJxKFoXK9M0ujM-xUISzBjBfAQv_4Frn0I33lZgxVE-BvxAF3sola2piz64VofP4u-JI3C-A6z2hX4PLhZvc4LQWEgJTL8B0ut2ng</recordid><startdate>20020901</startdate><enddate>20020901</enddate><creator>Zweygarth, E</creator><creator>Lopez-Rebollar, L.M. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.)</creator><creator>Meyer, P</creator><general>AOSIS (Pty) Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope></search><sort><creationdate>20020901</creationdate><title>In vitro isolation of equine piroplasms derived from Cape Mountain zebra (Equus zebra zebra) in South Africa</title><author>Zweygarth, E ; Lopez-Rebollar, L.M. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.) ; Meyer, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f256t-77f5cda4fc7b3098f520737c2783459f67391beeb308a4cb701b7b642dd6149e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>AFRIQUE DU SUD</topic><topic>Animals</topic><topic>Babesia - isolation & purification</topic><topic>BABESIA CABALLI</topic><topic>BABESIA EQUI</topic><topic>Babesiosis - diagnosis</topic><topic>Babesiosis - epidemiology</topic><topic>Babesiosis - veterinary</topic><topic>Carrier State - diagnosis</topic><topic>Carrier State - veterinary</topic><topic>CULTIVO IN VITRO</topic><topic>CULTURE IN VITRO</topic><topic>Equidae - parasitology</topic><topic>IN VITRO CULTURE</topic><topic>In Vitro Techniques</topic><topic>Parasitemia - diagnosis</topic><topic>Parasitemia - epidemiology</topic><topic>Parasitemia - veterinary</topic><topic>PIROPLASMEA</topic><topic>Seroepidemiologic Studies</topic><topic>SOUTH AFRICA</topic><topic>SUDAFRICA</topic><topic>THEILERIA</topic><topic>Theileria - isolation & purification</topic><topic>Theileriasis - diagnosis</topic><topic>Theileriasis - epidemiology</topic><topic>ZEBRA</topic><topic>ZEBRAS</topic><topic>ZEBRE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zweygarth, E</creatorcontrib><creatorcontrib>Lopez-Rebollar, L.M. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.)</creatorcontrib><creatorcontrib>Meyer, P</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Complete (ProQuest Database)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Journals (ProQuest Database)</collection><collection>ProQuest Biological Science Journals</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><jtitle>Onderstepoort journal of veterinary research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zweygarth, E</au><au>Lopez-Rebollar, L.M. (Agricultural Research Council, Onderstepoort (South Africa). Onderstepoort Veterinary Inst.)</au><au>Meyer, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro isolation of equine piroplasms derived from Cape Mountain zebra (Equus zebra zebra) in South Africa</atitle><jtitle>Onderstepoort journal of veterinary research</jtitle><addtitle>Onderstepoort J Vet Res</addtitle><date>2002-09-01</date><risdate>2002</risdate><volume>69</volume><issue>3</issue><spage>197</spage><epage>200</epage><pages>197-200</pages><issn>0030-2465</issn><eissn>2219-0635</eissn><coden>OJVRAZ</coden><abstract>Twenty blood samples of zebras (Equus zebra zebra) from the Karoo National Park and the Bontebok National Park in South Africa, all seropositive for Theileria equi, were subjected to in vitro culture to identify carrier animals and to isolate the parasites. Sixteen animals had a detectable parasitaemia in Giemsa-stained blood smears examined before culture initiation, the remaining four animals were identified as T. equi carriers by in vitro culture. Cultures were initiated either in an oxygen-reduced gas mixture or in a 5% CO2-in-air atmosphere. Out of the 20 blood samples, 12 cultures of T. equi and two cultures of T. equi mixed with Babesia caballi were established. None of the four animals seropositive for B. caballi could be identified as carrier animals, whereas two seronegative samples became culture-positive for B. caballi.</abstract><cop>South Africa</cop><pub>AOSIS (Pty) Ltd</pub><pmid>12356165</pmid><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0030-2465 |
| ispartof | Onderstepoort journal of veterinary research, 2002-09, Vol.69 (3), p.197-200 |
| issn | 0030-2465 2219-0635 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_72144215 |
| source | Publicly Available Content Database |
| subjects | AFRIQUE DU SUD Animals Babesia - isolation & purification BABESIA CABALLI BABESIA EQUI Babesiosis - diagnosis Babesiosis - epidemiology Babesiosis - veterinary Carrier State - diagnosis Carrier State - veterinary CULTIVO IN VITRO CULTURE IN VITRO Equidae - parasitology IN VITRO CULTURE In Vitro Techniques Parasitemia - diagnosis Parasitemia - epidemiology Parasitemia - veterinary PIROPLASMEA Seroepidemiologic Studies SOUTH AFRICA SUDAFRICA THEILERIA Theileria - isolation & purification Theileriasis - diagnosis Theileriasis - epidemiology ZEBRA ZEBRAS ZEBRE |
| title | In vitro isolation of equine piroplasms derived from Cape Mountain zebra (Equus zebra zebra) in South Africa |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T22%3A20%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20vitro%20isolation%20of%20equine%20piroplasms%20derived%20from%20Cape%20Mountain%20zebra%20(Equus%20zebra%20zebra)%20in%20South%20Africa&rft.jtitle=Onderstepoort%20journal%20of%20veterinary%20research&rft.au=Zweygarth,%20E&rft.date=2002-09-01&rft.volume=69&rft.issue=3&rft.spage=197&rft.epage=200&rft.pages=197-200&rft.issn=0030-2465&rft.eissn=2219-0635&rft.coden=OJVRAZ&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E246376681%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-f256t-77f5cda4fc7b3098f520737c2783459f67391beeb308a4cb701b7b642dd6149e3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=195083085&rft_id=info:pmid/12356165&rfr_iscdi=true |