Expression and regulation of the gene for arginase I in mouse salivary glands: requirement of CCAAT/enhancer-binding protein alpha for the expression in the parotid gland

Arginase in salivary glands is potentially involved in the synthesis of proline, glutamate, and polyamines that play specific physiological roles in the glands, and also in depletion of arginine in the oral cavity to protect teeth from microorganisms. We detected protein and mRNA for the type I isof...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 2002-10, Vol.132 (4), p.621-627
Main Authors: Akiba, Tsugino, Kuroiwa, Namiko, Shimizu-Yabe, Atsuko, Iwase, Katsuro, Hiwasa, Takaki, Yokoe, Hidetaka, Kubosawa, Hitoshi, Kageyama, Ryoichiro, Darlington, Gretchen J, Mori, Masataka, Tanzawa, Hideki, Takiguchi, Masaki, Dahrington, Gretchen J
Format: Article
Language:English
Subjects:
Animals
Arginase - biosynthesis
Arginase - genetics
Blotting, Northern
Blotting, Western
CCAAT-Enhancer-Binding Protein-alpha - genetics
CCAAT-Enhancer-Binding Protein-alpha - metabolism
Female
Gene Expression Regulation, Enzymologic
Gene Targeting
Immunohistochemistry
Isoenzymes - biosynthesis
Isoenzymes - genetics
Male
Mice
Mice, Transgenic
Parotid Gland - enzymology
Parotid Gland - physiology
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
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Summary:Arginase in salivary glands is potentially involved in the synthesis of proline, glutamate, and polyamines that play specific physiological roles in the glands, and also in depletion of arginine in the oral cavity to protect teeth from microorganisms. We detected protein and mRNA for the type I isoform of arginase in mouse salivary glands. Enzymes of the arginine-biosynthetic pathway were also detected. Immunohistochemical analysis revealed that arginase I was enriched in the striated duct, and was also present in the acinus, demilune and granulated duct. Mice with targeted disruption of the gene for C/EBPalpha, which is a transcription factor essential for expression of the arginase I gene in the liver, showed dramatically reduced immunoreactivity for arginase I in the parotid gland but not in the submandibular and sublingual glands. Therefore, C/EBPalpha is specifically required for expression of the arginase I gene in the parotid gland.
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a003265