Cryopreserved Cells Isolated from the Adult Canine Olfactory Bulb Are Capable of Extensive Remyelination Following Transplantation into the Adult Rat CNS

Naturally occurring spinal cord injury in dogs provides a potentially powerful intermediate model for testing the efficacy of therapeutic strategies developed in experimental rodent models before phase 1 trials in human patients. A particularly promising strategy involves transplantation of olfactor...

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Bibliographic Details
Published in:Experimental neurology 2002-08, Vol.176 (2), p.402-406
Main Authors: Smith, P.M., Lakatos, A., Barnett, S.C., Jeffery, N.D., Franklin, R.J.M.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain Tissue Transplantation - methods
Cell Separation
Cryopreservation
Demyelinating Diseases - pathology
Demyelinating Diseases - therapy
Development. Senescence. Regeneration. Transplantation
Disease Models, Animal
Dogs
Feasibility Studies
Fundamental and applied biological sciences. Psychology
Graft Survival
Immunohistochemistry
Myelin Sheath - metabolism
Nerve Regeneration - physiology
Olfactory Bulb - cytology
Olfactory Bulb - transplantation
Rats
Rats, Sprague-Dawley
Spinal Cord - cytology
Spinal Cord - pathology
Vertebrates: nervous system and sense organs
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Summary:Naturally occurring spinal cord injury in dogs provides a potentially powerful intermediate model for testing the efficacy of therapeutic strategies developed in experimental rodent models before phase 1 trials in human patients. A particularly promising strategy involves transplantation of olfactory ensheathing cells (OECs) that both promote axon regeneration and generate new myelin sheaths. As a first step in developing OEC transplantation in the canine intermediate model we describe the isolation, purification, and characterization of OECs from adult dog olfactory bulb. We also show that the canine OEC behaves in a manner similar to its rodent counterpart following transplantation into demyelinating lesions in rat spinal cord and that these properties are retained following cryopreservation.
ISSN:0014-4886
1090-2430
DOI:10.1006/exnr.2002.7936