Novel approach to fabricate porous gelatin–siloxane hybrids for bone tissue engineering

Porous and bioactive gelatin–siloxane hybrids were successfully synthesized by using a combined sol–gel processing, post-gelation soaking, and freeze-drying process to provide a novel kind of materials in the developments and optimization of bone tissue engineering. The pore sizes of the hybrids can...

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Bibliographic Details
Published in:Biomaterials 2002-12, Vol.23 (24), p.4765-4773
Main Authors: Ren, Lei, Tsuru, Kanji, Hayakawa, Satoshi, Osaka, Akiyoshi
Format: Article
Language:English
Subjects:
3T3 Cells
Alkaline Phosphatase - metabolism
Animals
Bioactivity
Bone Substitutes - chemistry
Calcium - metabolism
Cell culture
Cell Differentiation
Cell Division
Gelatin
Gelatin - chemistry
Ions
Lyophilization
Mice
Microscopy, Electron, Scanning
Scaffold
Siloxane
Siloxanes - chemistry
Temperature
Time Factors
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Summary:Porous and bioactive gelatin–siloxane hybrids were successfully synthesized by using a combined sol–gel processing, post-gelation soaking, and freeze-drying process to provide a novel kind of materials in the developments and optimization of bone tissue engineering. The pore sizes of the hybrids can be well controlled by varying the freezing temperature. The scaffolds were soaked in a simulated body fluid (SBF) up to 14 days to evaluate the in vitro bioactivity. The Ca 2+-containing scaffolds showed in vitro bioactivity as they biomimetically deposited apatite, but the Ca 2+-free scaffolds failed. Cytotoxicity and cytocompatibility of those scaffolds and their extracts were monitored by the MC3T3-E1 cell responses, including the cell proliferation and the alkaline phosphatase activity. It was demonstrated that appropriate incorporation of Ca 2+ ions stimulated osteoblast proliferation and differentiation in vitro.
ISSN:0142-9612
1878-5905
DOI:10.1016/S0142-9612(02)00226-0