Species identification and characterization of an Acanthamoeba strain from human cornea

The isoenzyme pattern of an Acanthamoeba, stock H-1, isolated from a patient with keratitis (Krankenhaus Heidberg, Hamburg) was compared with that of two strains of A. quina-A. lugdunensis (302-2, 312-1), two stocks of A. lenticulata (45, 89-1) and one strain of A. rhysodes (302-1). The isolated sto...

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Bibliographic Details
Published in:Parasitology research (1987) 1991-01, Vol.77 (6), p.469-474
Main Authors: MATIAS, R, SCHOTTELIUS, J, RADDATZ, C. F, MICHEL, R
Format: Article
Language:English
Subjects:
Acanthamoeba - classification
Acanthamoeba - enzymology
Acanthamoeba - ultrastructure
Acanthamoeba Keratitis - parasitology
Adult
Agglutination Tests
Animals
Biological and medical sciences
Biological Assay
Cell Nucleus - ultrastructure
Cornea - parasitology
Fundamental and applied biological sciences. Psychology
Humans
Isoenzymes - analysis
Male
Mice
Protozoa
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Summary:The isoenzyme pattern of an Acanthamoeba, stock H-1, isolated from a patient with keratitis (Krankenhaus Heidberg, Hamburg) was compared with that of two strains of A. quina-A. lugdunensis (302-2, 312-1), two stocks of A. lenticulata (45, 89-1) and one strain of A. rhysodes (302-1). The isolated stock showed glucose-6-phosphate dehydrogenase (G-6-PDH), beta-hydroxybutyric dehydrogenase (beta-HBDH), alcohol dehydrogenase (ADH) and superoxide dismutase (SOD) isoenzyme patterns similar to those of A. quina-A. lugdunensis but their acid phosphatase (AP) patterns differed. Furthermore, cyst morphology showed that the patient-isolated stock belongs to group II of the taxonomic classification of Acanthamoeba. This stock was not thermophilic and exhibited non-pathogenic properties after its intranasal instillation into NMRI mice, whereas it killed BALB/c mice. Immunofluorescent studies revealed the presence of antibodies against Acanthamoeba in the patient's serum. Immunoblotting experiments showed that a 45-kDa protein reacted with this serum. Such an antigen was also detected in A. quina-A. lugdunensis and A. lenticulata. Lectin reactions with Canavalia ensiformis, Ricinus communis-120, Lotus tetragonolobus, Ulex europaeus I, Helix pomatia, Arachis hypogaea, Triticum vulgaris, Glycine maxima, Bauhinia purpurea and Mycoplasma gallisepticum demonstrated that only the A. lenticulata stocks could not be distinguished and that the H-1 stock was more similar to the A. lugdunensis 302-2 strain than to the other acanthamoebae.
ISSN:0932-0113
1432-1955
DOI:10.1007/BF00928411