Characterization of a Neu/c-erbB-2 Protein-Specific Activating Factor

The neu oncogene encodes a tyrosine kinase with growth factor receptor-like properties. A neu protein-specific activating factor (NAF) was partially purified from medium conditioned by the transformed human T-cell line ATL-2. NAF was able to stimulate the tyrosine-specific kinase activity of the neu...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1991-10, Vol.88 (19), p.8582-8586
Main Authors: Dobashi, Kunio, Davis, James G., Mikami, Yasunori, Freeman, Jonathan K., Hamuro, Junji, Greene, Mark I.
Format: Article
Language:English
Subjects:
Antibodies
Biochemistry
Biological and medical sciences
Cell Division - drug effects
Cell growth
Cell Line
Cell lines
Cell physiology
Dimerization
Down-Regulation
Enzyme Activation
Fundamental and applied biological sciences. Psychology
Humans
Immunoprecipitation
Internalization
Ligands
Macromolecular Substances
Molecular and cellular biology
Monoclonal antibodies
Phosphotyrosine
Protein-Tyrosine Kinases - metabolism
Proto-Oncogene Proteins - metabolism
Receptor Aggregation
Receptor, ErbB-2
Receptors
Responses to growth factors, tumor promotors, other factors
T-Lymphocytes - chemistry
Tyrosine - analogs & derivatives
Tyrosine - metabolism
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Summary:The neu oncogene encodes a tyrosine kinase with growth factor receptor-like properties. A neu protein-specific activating factor (NAF) was partially purified from medium conditioned by the transformed human T-cell line ATL-2. NAF was able to stimulate the tyrosine-specific kinase activity of the neu protein (p185neu), induce dimerization and internalization, and increase the growth of cells bearing the neu protein. The effects of NAF were mediated by an interaction with the p185neuextracellular domain. NAF had no effect on the epidermal growth factor receptor kinase activity and no effect on cells that express that receptor. Further analysis of NAF and of other recently described neu protein-activating activities should help clarify the role of the neu protein in cell growth and transformation
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.88.19.8582