Transcriptome analysis of all two‐component regulatory system mutants of Escherichia coli K‐12

• Published in: Molecular microbiology 2002-10, Vol.46 (1), p.281-291
• Main Authors: Oshima, Taku, Aiba, Hirofumi, Masuda, Yasushi, Kanaya, Shigehiko, Sugiura, Masahito, Wanner, Barry L., Mori, Hirotada, Mizuno, Takeshi
• Format: Article
• Language: English
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Escherichia coli - genetics; Escherichia coli - growth & development; Escherichia coli - physiology; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Mutation; Oligonucleotide Array Sequence Analysis; Proteome; Signal Transduction; Transcription, Genetic
• ISSN: 0950-382X; 1365-2958
• DOI: 10.1046/j.1365-2958.2002.03170.x

Summary We have systematically examined the mRNA profiles of 36 two‐component deletion mutants, which include all two‐component regulatory systems of Escherichia coli, under a single growth condition. DNA microarray results revealed that the mutants belong to one of three groups based on their gene expression profiles in Luria–Bertani broth under aerobic conditions: (i) those with no or little change; (ii) those with significant changes; and (iii) those with drastic changes. Under these conditions, the anaeroresponsive ArcB/ArcA system, the osmoresponsive EnvZ/OmpR system and the response regulator UvrY showed the most drastic changes. Cellular functions such as flagellar synthesis and expression of the RpoS regulon were affected by multiple two‐component systems. A high correlation coefficient of expression profile was found between several two‐component mutants. Together, these results support the view that a network of functional interactions, such as cross‐regulation, exists between different two‐component systems. The compiled data are avail‐able at our website (http:ecoli.aist‐nara.ac.jpxpanalysis 2components).