Recirculating, retrograde heart perfusion according to Langendorff as a tool in the evaluation of drug-induced cardiomyopathy : effects of a high lipid diet

A recirculating, retrograde heart perfusion according to Langendorff is described as a method for the evaluation of cardiomyopathy as an untoward side effect of a high lipid diet (addition of 10% and 25% corn oil to rat maintenance feed) in female rats. The use of glucose (5 mM) or palmitate (0.5 mM...

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Bibliographic Details
Published in:Archives of toxicology 1991-01, Vol.65 (6), p.474-479
Main Authors: BACHMANN, E, WEBER, E
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cardiomyopathies - etiology
Creatine Kinase - metabolism
Dietary Fats - adverse effects
Energy Metabolism
Female
General aspects. Methods
Glycogen - analysis
L-Lactate Dehydrogenase - metabolism
Medical sciences
Myocardium - metabolism
Perfusion
Phosphocreatine - analysis
Pyruvates - analysis
Pyruvic Acid
Rats
Toxicology
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Summary:A recirculating, retrograde heart perfusion according to Langendorff is described as a method for the evaluation of cardiomyopathy as an untoward side effect of a high lipid diet (addition of 10% and 25% corn oil to rat maintenance feed) in female rats. The use of glucose (5 mM) or palmitate (0.5 mM/0.1 mM BSA) as substrates during a 2-h perfusion period, and their effects on heart metabolism of control-, LL- and HL-diet fed animals were evaluated. Substrate uptake, LDH release and adenine nucleotides, creatine phosphate, creatine, lactate, pyruvate, glucose-6-phosphate, glycogen, triglyceride and phospholipid content were determined in heart tissue.
ISSN:0340-5761
1432-0738
DOI:10.1007/BF01977359