Serial propagation of porcine enteric calicivirus in a continuous cell line. Effect of medium supplementation with intestinal contents or enzymes

A porcine enteric calicivirus (PEC) was adapted to serial propagation in a continuous porcine kidney cell line (LLC-PK) by inclusion of an intestinal contents preparation (ICP), derived from uninfected gnotobiotic pigs, in the maintenance medium. Infected cells were detected by immunofluorescent sta...

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Bibliographic Details
Published in:Archives of virology 1991-01, Vol.120 (1-2), p.115-122
Main Authors: PARWANI, A. V, FLYNN, W. T, GADFIELD, K. L, SAIF, L. J
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Caliciviridae - growth & development
Cell Line
Culture Media
Fundamental and applied biological sciences. Psychology
Gastrointestinal Contents
Intestines - enzymology
Kidney - cytology
Kidney - microbiology
Microbiology
porcine enteric calicivirus
Serial Passage
Swine - microbiology
Techniques used in virology
Virology
Virus Cultivation
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Summary:A porcine enteric calicivirus (PEC) was adapted to serial propagation in a continuous porcine kidney cell line (LLC-PK) by inclusion of an intestinal contents preparation (ICP), derived from uninfected gnotobiotic pigs, in the maintenance medium. Infected cells were detected by immunofluorescent staining of cell monolayers. Attempts to propagate PEC in the presence of various intestinal enzymes of porcine origin, bacterial protease, bovine ICP, avian ICP or without medium supplementation were unsuccessful.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01310954