Generation of multicolored, prestained molecular weight markers for gel electrophoresis

Molecular weight marker proteins are routinely used in sodium dodecyl sulfate‐polyacrylamide gel electrophoresis to estimate the relative molecular mass of specific proteins within a sample. This report describes a simple procedure for the generation of multicolored molecular weight proteins using a...

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Bibliographic Details
Published in:Electrophoresis 2002-09, Vol.23 (19), p.3262-3265
Main Authors: Compton, Mark M., Lapp, Stacey A., Pedemonte, Ron
Format: Article
Language:English
Subjects:
Color
Colored markers
Coloring Agents - chemistry
Electrophoresis, Polyacrylamide Gel - methods
Molecular Weight
Prestained markers
Reactive dyes
Reference Standards
Remazol dyes
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
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Summary:Molecular weight marker proteins are routinely used in sodium dodecyl sulfate‐polyacrylamide gel electrophoresis to estimate the relative molecular mass of specific proteins within a sample. This report describes a simple procedure for the generation of multicolored molecular weight proteins using a variety of Remazol‐reactive textile dyes. These multicolored proteins provide a set of unambiguous markers for gel electrophoresis. Furthermore, the colored markers can be used in conjunction with Western blotting techniques to provide a visual display of marker proteins on the transfer membrane.
ISSN:0173-0835
1522-2683
DOI:10.1002/1522-2683(200210)23:19<3262::AID-ELPS3262>3.0.CO;2-8