A new class of tetraspanins in fungi

Tetraspanins are animal proteins involved in membrane complexes that are involved in cell adhesion, differentiation, and motility. The PLS1 gene from rice blast fungus Magnaporthe grisea encodes a protein (Pls1p) structurally related to tetraspanins that is required for pathogenicity. In Botrytis ci...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2002-10, Vol.297 (5), p.1197-1204
Main Authors: Gourgues, M, Clergeot, P.-H, Veneault, C, Cots, J, Sibuet, S, Brunet-Simon, A, Levis, C, Langin, T, Lebrun, M.-H
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Blotting, Southern
Botrytis - metabolism
Botrytis cinerea
Cloning, Molecular
Colletotrichum lindemuthianum
DNA, Complementary - metabolism
Exons
Expressed Sequence Tags
Fungal Proteins - chemistry
Fungal Proteins - classification
Fungal Proteins - genetics
Fungi - metabolism
Introns
Magnaporthe - metabolism
Magnaporthe grisea
Membrane Proteins - chemistry
Membrane Proteins - classification
Membrane Proteins - genetics
Molecular Sequence Data
Neurospora crassa
Neurospora crassa - metabolism
Phylogeny
PLS1
Polymerase Chain Reaction
Protein Structure, Tertiary
Sequence Homology, Amino Acid
Software
Tetraspanin
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Summary:Tetraspanins are animal proteins involved in membrane complexes that are involved in cell adhesion, differentiation, and motility. The PLS1 gene from rice blast fungus Magnaporthe grisea encodes a protein (Pls1p) structurally related to tetraspanins that is required for pathogenicity. In Botrytis cinerea public sequences, we identified an EST homologous to PLS1. Using degenerated oligonucleotides, we amplified sequences homologous to PLS1 in fungi Colletotrichum lindemuthianum and Neurospora crassa. Analysis of N. crassa and M. grisea genome sequences revealed the presence of a single tetraspanin gene. Thus, fungi differ from animals, which contain between 20 and 37 paralogous tetraspanin genes. Fungal proteins encoded by BcPLS1, ClPLS1, and NcPLS1 display all the structural hallmarks of tetraspanins (predicted topology with four transmembrane domains, extra- and intracellular loops; conserved cysteine-based patterns in second extracellular loop). Phylogenetic analysis suggests that these genes define a new family of orthologous genes encoding fungal-specific tetraspanins.
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(02)02355-0