Semi-automation of the polymerase chain reaction for laboratory confirmation of meningococcal disease

Demand for accurate high-throughput detection and characterisation of medically important bacteria has increased dramatically within research and clinical laboratories. Liquid-handling robots have been developed to achieve high levels of accuracy and reproducibility. Assay automation can play a key...

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Bibliographic Details
Published in:British journal of biomedical science 2002-01, Vol.59 (3), p.137-140
Main Authors: Diggle, M.A., Clarke, S.C.
Format: Article
Language:English
Subjects:
Automation
Bacteriological Techniques - instrumentation
DNA, Bacterial - analysis
Humans
Meningitis, Meningococcal - diagnosis
Meningococcus
Neisseria meningitidis
Neisseria meningitidis - isolation & purification
Polymerase chain reaction
Polymerase Chain Reaction - instrumentation
Robotics - instrumentation
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Summary:Demand for accurate high-throughput detection and characterisation of medically important bacteria has increased dramatically within research and clinical laboratories. Liquid-handling robots have been developed to achieve high levels of accuracy and reproducibility. Assay automation can play a key role in the modern diagnostic laboratory and the data presented here shows that automated PCR is comparable with manual methods. Importantly, automation is preferred when high-quality results cannot be guaranteed using manual methods. This is particularly important when results are required quickly for public health management.
ISSN:0967-4845
2474-0896
DOI:10.1080/09674845.2002.11783649