F- and V-type ATPases in the hyperthermophilic bacterium Thermotoga neapolitana

• Published in: Extremophiles : life under extreme conditions 2002-10, Vol.6 (5), p.369-375
• Main Authors: IIDA, Toshii, INATOMI, Ken-Ichi, KAMAGATA, Yoichi, MARUYAMA, Tadashi
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Bacterial Proteins - genetics; Bacterial Proteins - isolation & purification; Bacterial Proteins - metabolism; Bacteriology; Biological and medical sciences; Blotting, Western; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genetics; Gram-Negative Anaerobic Straight, Curved, and Helical Rods - enzymology; Gram-Negative Anaerobic Straight, Curved, and Helical Rods - genetics; Hot Temperature; Membrane Proteins - genetics; Membrane Proteins - isolation & purification; Membrane Proteins - metabolism; Metabolism. Enzymes; Microbiology; Molecular Sequence Data; Operon; Phylogeny; Proton-Translocating ATPases - genetics; Proton-Translocating ATPases - isolation & purification; Proton-Translocating ATPases - metabolism; Sequence Homology, Amino Acid; Solubility; Space life sciences; Vacuolar Proton-Translocating ATPases - genetics; Vacuolar Proton-Translocating ATPases - isolation & purification; Vacuolar Proton-Translocating ATPases - metabolism
• ISSN: 1431-0651; 1433-4909
• DOI: 10.1007/s00792-002-0266-7

Two gene clusters encoding F- or V-type ATPases were found in genomic DNA of the hyperthermophilic bacterium Thermotoga neapolitana. The subunit genes of each ATPase formed an operon. While the gene arrangement in the operon of the F-type ATPase resembled those in eukaryotic organelles and bacteria, that of the V-type ATPase was different from those reported for archaea, bacteria, or eukaryotes. Both ATPases were found to be expressed in the cells of T. neapolitana by Western blot analysis. Although V-type ATPase could not be rendered soluble, F-type ATPase was solubilized with 1% Triton X-100 and characterized. This is the first report of the coexistence of both F- and V-type ATPases in hyperthermophilic bacteria. It has recently been shown by a genome analysis that Thermotoga maritima has no V-type ATPase gene cluster but does have an F-type ATPase gene cluster; however, part of a gene for the D-subunit of the V-type ATPase gene has been reported in the T. maritima genome. Evolution of the two types of ATPases in Thermotoga is discussed.