Dedifferentiation of epidermal cells to stem cells in vivo

The effects of growth factors on wound healing have been studied extensively; however epidermal regeneration is not fully understood. We treated eight patients with leg ulcers with recombinant human epidermal growth factor (rhEGF) and compared biopsies of regenerating epidermis with those of control...

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Published in:The Lancet (British edition) 2001-09, Vol.358 (9287), p.1067-1068
Main Authors: Fu, Xiaobing, Sun, Xiaoqing, Li, Xiaokun, Sheng, Zhiyong
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Biopsy
Case-Control Studies
Cell cycle
Cell Differentiation
Cell division
Epidermal growth factor
Epidermal Growth Factor - therapeutic use
Epidermis
Growth factors
Humans
Immunohistochemistry
Injuries
Integrin beta1 - biosynthesis
Islands
Keratin
Keratins - biosynthesis
Leg Ulcer - drug therapy
Leg Ulcer - metabolism
Leg Ulcer - pathology
Leg ulcers
Medical research
Medical sciences
Patients
Pharmacology. Drug treatments
Regeneration
Reversion
Skin - metabolism
Skin - pathology
Skin, nail, hair, dermoskeleton
Stem cells
Stem Cells - physiology
Ulcers
Wound healing
Wound Healing - drug effects
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creator Fu, Xiaobing
Sun, Xiaoqing
Li, Xiaokun
Sheng, Zhiyong
description The effects of growth factors on wound healing have been studied extensively; however epidermal regeneration is not fully understood. We treated eight patients with leg ulcers with recombinant human epidermal growth factor (rhEGF) and compared biopsies of regenerating epidermis with those of controls who did not receive rhEGF. We used immunohistochemistry to identify cells expressing keratin 19 and β1 integrin in regenerated epidermis from patients and controls. Patients treated with rhEGF had stem cells in the spinous and granular layers of regenerated epidermis. Histological analysis showed that these stem cells had reverted from differentiated to undifferentiated stem cells. Our findings provide evidence for epidermal cell reversion.
doi_str_mv 10.1016/S0140-6736(01)06202-X
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We treated eight patients with leg ulcers with recombinant human epidermal growth factor (rhEGF) and compared biopsies of regenerating epidermis with those of controls who did not receive rhEGF. We used immunohistochemistry to identify cells expressing keratin 19 and β1 integrin in regenerated epidermis from patients and controls. Patients treated with rhEGF had stem cells in the spinous and granular layers of regenerated epidermis. Histological analysis showed that these stem cells had reverted from differentiated to undifferentiated stem cells. Our findings provide evidence for epidermal cell reversion.</description><identifier>ISSN: 0140-6736</identifier><identifier>EISSN: 1474-547X</identifier><identifier>DOI: 10.1016/S0140-6736(01)06202-X</identifier><identifier>PMID: 11589942</identifier><identifier>CODEN: LANCAO</identifier><language>eng</language><publisher>London: Elsevier Ltd</publisher><subject>Adult ; Biological and medical sciences ; Biopsy ; Case-Control Studies ; Cell cycle ; Cell Differentiation ; Cell division ; Epidermal growth factor ; Epidermal Growth Factor - therapeutic use ; Epidermis ; Growth factors ; Humans ; Immunohistochemistry ; Injuries ; Integrin beta1 - biosynthesis ; Islands ; Keratin ; Keratins - biosynthesis ; Leg Ulcer - drug therapy ; Leg Ulcer - metabolism ; Leg Ulcer - pathology ; Leg ulcers ; Medical research ; Medical sciences ; Patients ; Pharmacology. 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subjects Adult
Biological and medical sciences
Biopsy
Case-Control Studies
Cell cycle
Cell Differentiation
Cell division
Epidermal growth factor
Epidermal Growth Factor - therapeutic use
Epidermis
Growth factors
Humans
Immunohistochemistry
Injuries
Integrin beta1 - biosynthesis
Islands
Keratin
Keratins - biosynthesis
Leg Ulcer - drug therapy
Leg Ulcer - metabolism
Leg Ulcer - pathology
Leg ulcers
Medical research
Medical sciences
Patients
Pharmacology. Drug treatments
Regeneration
Reversion
Skin - metabolism
Skin - pathology
Skin, nail, hair, dermoskeleton
Stem cells
Stem Cells - physiology
Ulcers
Wound healing
Wound Healing - drug effects
title Dedifferentiation of epidermal cells to stem cells in vivo
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