Characterization of the nm23-M2, nm23-M3 and nm23-M4 mouse genes: comparison with their human orthologs

• Published in: Gene 2002-08, Vol.296 (1-2), p.87-97
• Main Authors: Massé, K, Dabernat, S, Bourbon, P-M, Larou, M, Amrein, L, Barraud, P, Perel, Y, Camara, M, Landry, M, Lacombe, M-L, Daniel, J-Y
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Blotting, Northern; Cloning, Molecular; DNA - chemistry; DNA - genetics; DNA, Complementary - chemistry; DNA, Complementary - genetics; Embryo, Mammalian - enzymology; Embryo, Mammalian - metabolism; Exons; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Genes - genetics; Humans; In Situ Hybridization; Introns; Isoenzymes - genetics; Mice; Molecular Sequence Data; Monomeric GTP-Binding Proteins - genetics; NM23 Nucleoside Diphosphate Kinases; Nucleoside-Diphosphate Kinase - genetics; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Transcription Factors - genetics; Transcription Initiation Site
• ISSN: 0378-1119
• DOI: 10.1016/S0378-1119(02)00836-3

The nm23 gene family is thought to be involved in physiopathological processes such as growth, differentiation and cancer promotion, progression or metastasis. We report here the mouse nm23-M3 and nm23-M4 complementary DNA sequences and the genomic cloning, characterization and tissue expression pattern of the nm23-M2, nm23-M3 and nm23-M4 genes, in comparison with their human and rat orthologs and with the human nm23-H1 and mouse nm23-M1 genes. The organization and structure of the members of this gene family are remarkably similar in human and rodents. Accordingly, the striking similarities between the human and mouse nm23 genes enable the use of mouse transgenic and knock-out models for studying the role of nucleoside diphosphate kinase isoforms in human physiopathology.