Modulation of the immune response mediated by oral transgene administration of IL-10

In the current study, we analyze the immunomodulatory effect of oral transgene administration of IL-10 using a mice model of viral inflammation. Salmonella harboring a plasmid encoding the IL-10 gene (SLIL10) was administered by the oral route together with Salmonella carrying a plasmid encoding the...

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Bibliographic Details
Published in:Cellular immunology 2002-03, Vol.216 (1), p.73-81
Main Authors: Elias, F, Flo, J
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
Antibody Formation
Disease Models, Animal
DNA immunization
Down-Regulation
Female
Herpes Genitalis - prevention & control
Herpes Simplex - immunology
Herpesvirus 2, Human - genetics
Herpesvirus 2, Human - immunology
HSV
IL-10
Immune suppression
Immunity, Cellular
Injections, Intramuscular
Interleukin-10 - genetics
Interleukin-10 - immunology
Mice
Mice, Inbred BALB C
Plasmids - genetics
Salmonella
Salmonella - genetics
Transformation, Bacterial
Transgenes
Vaccination
Vaccines, Synthetic - genetics
Vaccines, Synthetic - immunology
Viral Envelope Proteins - genetics
Viral Envelope Proteins - immunology
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Summary:In the current study, we analyze the immunomodulatory effect of oral transgene administration of IL-10 using a mice model of viral inflammation. Salmonella harboring a plasmid encoding the IL-10 gene (SLIL10) was administered by the oral route together with Salmonella carrying a plasmid encoding the glycoprotein D or B (SLgD, SLgB) of Herpes simplex virus type 2 (HSV-2). This resulted in a high inhibition of the cellular and human immune response against the viral proteins. When mice immunized against the HSV proteins were challenged with 10 lethal doses of HSV-2 by the intravaginal route, only those that had also received SLIL10 showed severe lesions and died. When Salmonella harboring pIL10 was administered orally to mice immunized by the intramuscular route with a plasmid encoding gD, inhibition of cellular and humoral immune responses were also observed but to a lesser extent than with oral immunization. By means of adoptive transfer experiments and in vitro experiments, we have subsequently determined that the mechanism possibly involved in the inhibition of the immune response could be a reduced antigenic presentation when mice receive SLIL10 that induced a state of anergy on specific T lymphocytes.
ISSN:0008-8749
1090-2163
DOI:10.1016/S0008-8749(02)00507-5