Genetically encoded optical sensors of neuronal activity and cellular function

Fluorescent proteins (FPs) have been engineered to produce an optical report in response to cellular signals. FP fluorescence can be made directly sensitive to the chemical environment, via specific mutations of or around the chromophore. Alternatively, FPs can be made indirectly sensitive to cellul...

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Bibliographic Details
Published in:Current Opinion in Neurobiology 2001-10, Vol.11 (5), p.601-607
Main Authors: Guerrero, Giovanna, Isacoff, Ehud Y
Format: Article
Language:English
Subjects:
Animals
biosensor
DsRed
fluorescence
Genetic Engineering - methods
Green Fluorescent Protein
Green Fluorescent Proteins
Humans
imaging
Indicators and Reagents
Luminescent Proteins - analysis
Luminescent Proteins - genetics
Neurons - chemistry
Neurons - physiology
optical
Optics and Photonics
sensor
Signal Transduction - genetics
signaling
spectroscopy
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Summary:Fluorescent proteins (FPs) have been engineered to produce an optical report in response to cellular signals. FP fluorescence can be made directly sensitive to the chemical environment, via specific mutations of or around the chromophore. Alternatively, FPs can be made indirectly sensitive to cellular signals by their fusion to ‘detector’ proteins that respond to specific cellular signals with structural rearrangements that act on the FP to alter fluorescence. These optical sensors of membrane voltage, neurotransmitter release, and intracellular messengers, including powerful new sensors of Ca 2+, cyclic nucleotides and nitric oxide, are likely to provide new insights into the workings of cellular signals and of information processing in neural circuits.
ISSN:0959-4388
1873-6882
DOI:10.1016/S0959-4388(00)00256-7