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Genetically encoded optical sensors of neuronal activity and cellular function
Fluorescent proteins (FPs) have been engineered to produce an optical report in response to cellular signals. FP fluorescence can be made directly sensitive to the chemical environment, via specific mutations of or around the chromophore. Alternatively, FPs can be made indirectly sensitive to cellul...
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| Published in: | Current Opinion in Neurobiology 2001-10, Vol.11 (5), p.601-607 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c392t-43690720f2458ba3c37a27827bb477ed88403267cb9868ca2158a969b4f0dc53 |
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| cites | cdi_FETCH-LOGICAL-c392t-43690720f2458ba3c37a27827bb477ed88403267cb9868ca2158a969b4f0dc53 |
| container_end_page | 607 |
| container_issue | 5 |
| container_start_page | 601 |
| container_title | Current Opinion in Neurobiology |
| container_volume | 11 |
| creator | Guerrero, Giovanna Isacoff, Ehud Y |
| description | Fluorescent proteins (FPs) have been engineered to produce an optical report in response to cellular signals. FP fluorescence can be made directly sensitive to the chemical environment, via specific mutations of or around the chromophore. Alternatively, FPs can be made indirectly sensitive to cellular signals by their fusion to ‘detector’ proteins that respond to specific cellular signals with structural rearrangements that act on the FP to alter fluorescence. These optical sensors of membrane voltage, neurotransmitter release, and intracellular messengers, including powerful new sensors of Ca
2+, cyclic nucleotides and nitric oxide, are likely to provide new insights into the workings of cellular signals and of information processing in neural circuits. |
| doi_str_mv | 10.1016/S0959-4388(00)00256-7 |
| format | article |
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| ispartof | Current Opinion in Neurobiology, 2001-10, Vol.11 (5), p.601-607 |
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| language | eng |
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| source | Elsevier |
| subjects | Animals biosensor DsRed fluorescence Genetic Engineering - methods Green Fluorescent Protein Green Fluorescent Proteins Humans imaging Indicators and Reagents Luminescent Proteins - analysis Luminescent Proteins - genetics Neurons - chemistry Neurons - physiology optical Optics and Photonics sensor Signal Transduction - genetics signaling spectroscopy |
| title | Genetically encoded optical sensors of neuronal activity and cellular function |
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