Identification of commercial fish species belonging to the orders Pleuronectiformes and Gadiformes: Library of isoelectric focusing patterns

Isoelectric focusing in polyacrylamide gel was used to establish an identification archive of fish species belonging to the orders Pleuronectiformes, or flat fish, and Gadiformes, or gadoid fish. The 2 orders include species of different commercial value and interest that are frequently requested in...

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Bibliographic Details
Published in:Journal of AOAC International 2001-09, Vol.84 (5), p.1600-1607
Main Authors: TEPEDINO, Valentina, BERRINI, Anna, BORROMEO, Vitaliano, GAGGIOLI, Daniela, CANTONI, Carlo, MANZONI, Paolo, SECCHI, Camillo
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Electrophoresis, Polyacrylamide Gel
Fish and seafood industries
Fishes - metabolism
Flatfishes - metabolism
Food industries
Fundamental and applied biological sciences. Psychology
Hydrogen-Ion Concentration
Isoelectric Focusing
Meat - analysis
Species Specificity
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Summary:Isoelectric focusing in polyacrylamide gel was used to establish an identification archive of fish species belonging to the orders Pleuronectiformes, or flat fish, and Gadiformes, or gadoid fish. The 2 orders include species of different commercial value and interest that are frequently requested in European fish markets, but are susceptible to substitution either because they are morphologically similar or because they arrive on the markets already filleted or sliced. The sarcoplasmic protein profiles are species-specific and reproducible. The use of densitometry and image analysis coupled with a simple computer program overcomes the subjective evaluation of the patterns, making it possible to identify species correctly.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/84.5.1600