Validation of a UV spectrophotometric method for the determination of melatonine in solid dosage forms

The aim of the work described in this paper was to provide a fast, easy, inexpensive, precise, and accurate method for the determination of melatonine in solid pharmaceutical dosage forms. The developed method is based on a UV first-derivative spectrophotometric determination, which exhibits excelle...

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Bibliographic Details
Published in:Journal of AOAC International 2001-09, Vol.84 (5), p.1352-1357
Main Authors: PEREZ, Renato F, LEMUS, Igor G, BOCIC, Rony V, PEREZ, Mauricio V, GARCIA-MADRID, Rubén
Format: Article
Language:English
Subjects:
Analysis
Antioxidants - analysis
Biological and medical sciences
General pharmacology
Medical sciences
Melatonin - analysis
Pharmacology. Drug treatments
Reproducibility of Results
Solutions
Spectrophotometry, Ultraviolet
Tablets
Tryptophan - analysis
Ultrasonics
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Summary:The aim of the work described in this paper was to provide a fast, easy, inexpensive, precise, and accurate method for the determination of melatonine in solid pharmaceutical dosage forms. The developed method is based on a UV first-derivative spectrophotometric determination, which exhibits excellent linearity in aqueous solutions (r2 = 0.996) for analyte concentrations of 1.5-4.5 mg/dL within a pH range of 5-9. Neither excipients present in the formulation nor indole adulterants, such as tryptophan (up to 5%), interfere with the assay. A study of variation parameters showed that sonication temperature was the main factor for successful determination. At temperatures of
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/84.5.1352