Analysis of three lupane type triterpenoids in Helicteres angustifolia by high-performance liquid chromatography

Kang-chih-ma is the dried roots and stems of Helicteres angustifolia (Sterculiaceae) and a commonly used folk herbal drug in Taiwan. It possesses anti-dotal, analgesic, anti-inflammatory and anti-bacterial effects and is also known as a kind of tumor inhibitory plant. To evaluate the quality of H. a...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2001-12, Vol.26 (5), p.849-855
Main Authors: Chang, Yuan-Shiun, Ku, Yoe-Ray, Lin, Jer-Huei, Lu, Kuo-Liang, Ho, Li-Kang
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Chromatography, High Pressure Liquid
General pharmacology
Helicteres angustifolia
Indicators and Reagents
Malvaceae - chemistry
Mass Spectrometry
Medical sciences
Methyl helicterate
Pharmaceutical analysis
Pharmacology. Drug treatments
Plant Epidermis - chemistry
Plant Roots - chemistry
Plant Stems - chemistry
Plants, Medicinal - chemistry
Reference Standards
Solvents
Taiwan
Triterpenes - analysis
Wood
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Summary:Kang-chih-ma is the dried roots and stems of Helicteres angustifolia (Sterculiaceae) and a commonly used folk herbal drug in Taiwan. It possesses anti-dotal, analgesic, anti-inflammatory and anti-bacterial effects and is also known as a kind of tumor inhibitory plant. To evaluate the quality of H. angustifolia, a simple, rapid and accurate high-performance liquid chromatographic (HPLC) method was developed for the assay of three lupane type triterpenes: 3β-acetoxy-27-benzoyloxylup-20(29)-en-28-oic acid methyl ester (methyl helicterate), 3β-acetoxy-27-benzoyloxylup-20(29)-en-28-oic acid, and 3β-acetoxy-27-( p-hydroxyl) benzoyloxylup-20(29)-en-28-oic acid methyl ester. The present HPLC system used an Inertsil ODS-2 column by gradient elution with acetonitrile and water as the mobile phase and detected at UV 230 nm. Regression equations revealed good linear relationships (correlation coefficients: 0.9922–0.9997). The relative standard deviations of these three constituents ranged between 1.05–3.14% (intraday) and 2.12–4.38% (interday). The contents of these three constituents of the heartwood and the bark of the roots of H. angustifolia in five different samples have also been determined.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(01)00465-4