Determination of Cell Viability in Single or Mixed Samples Using Capillary Electrophoresis Laser-Induced Fluorescence Microfluidic Systems

The advent of high-efficiency microbial separations will have a profound effect on both chemistry and microbiology. For the first time, it appears that it may be possible to obtain qualitative and quantitative information on microbial systems with the accuracy, precision, speed, and throughput that...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2001-10, Vol.73 (19), p.4551-4557
Main Authors: Armstrong, Daniel W, He, Lingfeng
Format: Article
Language:English
Subjects:
Bacteria
Bacteriological methods and techniques used in bacteriology
Bacteriology
Bifidobacterium - cytology
Bifidobacterium - isolation & purification
Biological and medical sciences
Cells
Chemistry
Electrophoresis, Capillary - methods
Fluorescence
Fundamental and applied biological sciences. Psychology
Lactobacillus acidophilus - cytology
Lactobacillus acidophilus - isolation & purification
Lasers
Microbiological Techniques - methods
Microbiology
Saccharomyces cerevisiae - cytology
Saccharomyces cerevisiae - isolation & purification
Spectrophotometry, Ultraviolet - methods
Yeast
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Summary:The advent of high-efficiency microbial separations will have a profound effect on both chemistry and microbiology. For the first time, it appears that it may be possible to obtain qualitative and quantitative information on microbial systems with the accuracy, precision, speed, and throughput that currently is found for chemical systems. Recently it was suggested that an analytical separations-based approach for determining the viability of cells would be advantageous. The feasibility of such an approach is demonstrated using CE-LIF of two bacteria and yeast. The analytical procedures and figures of merit are outlined. High-throughput analyses and evaluation of microorganisms now appear to be possible.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac010449q