Identification of an Arabidopsis cDNA encoding a lipoyltransferase located in plastids

In plant cells, the pyruvate dehydrogenase (PDH) complex that requires lipoic acid as an essential coenzyme is located in plastids and mitochondria. The enzyme complex has to be lipoylated in both organelles. However, the lipoyltransferase located in plastids has not been reported. In this study, an...

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Bibliographic Details
Published in:FEBS letters 2001-10, Vol.506 (3), p.286-290
Main Authors: Wada, Miho, Yasuno, Rie, Wada, Hajime
Format: Article
Language:English
Subjects:
ACP, acyl carrier protein
Acyltransferases - chemistry
Acyltransferases - genetics
Acyltransferases - metabolism
Amino Acid Sequence
Arabidopsis - enzymology
Arabidopsis - genetics
Arabidopsis thaliana
Cloning, Molecular
DNA, Complementary
Genetic Complementation Test
GFP, green fluorescent protein
Lipoic acid
Lipoylation
Lipoyltransferase
Molecular Sequence Data
OGDH, 2-oxoglutarate dehydrogenase
ORF, open reading frame
PCR, polymerase chain reaction
PDH, pyruvate dehydrogenase
Plastid
Plastids - enzymology
Pyruvate dehydrogenase
RT-PCR, reverse transcription-PCR
Sequence Homology, Amino Acid
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Summary:In plant cells, the pyruvate dehydrogenase (PDH) complex that requires lipoic acid as an essential coenzyme is located in plastids and mitochondria. The enzyme complex has to be lipoylated in both organelles. However, the lipoyltransferase located in plastids has not been reported. In this study, an Arabidopsis thaliana LIP2p cDNA for a lipoyltransferase located in plastids has been identified. We have shown that this cDNA encodes a lipoyltransferase by demonstrating its ability to complement an Escherichia coli mutant lacking lipoyltransferase activity, and that LIP2p is targeted into chloroplasts. These findings suggest that LIP2p is located in plastids and responsible for lipoylation of the plastidial PDH complex.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(01)02932-5