Expression of endothelial cell adhesion molecules in neovascularized tissue

• Published in: Microcirculation (New York, N.Y. 1994) N.Y. 1994), 2000-08, Vol.7 (4), p.249-258
• Main Authors: Vallien, G, Langley, R, Jennings, S, Specian, R, Granger, D N
• Format: Article
• Language: English
• Subjects: Animals; Antigens, CD - biosynthesis; Antigens, CD - drug effects; Antigens, CD - immunology; Cell Adhesion Molecules - biosynthesis; Cell Adhesion Molecules - drug effects; Cell Adhesion Molecules - immunology; E-Selectin - biosynthesis; E-Selectin - drug effects; E-Selectin - immunology; Endothelium, Vascular - chemistry; Endothelium, Vascular - cytology; Endothelium, Vascular - drug effects; Immunohistochemistry; Intercellular Adhesion Molecule-1 - biosynthesis; Intercellular Adhesion Molecule-1 - drug effects; Intercellular Adhesion Molecule-1 - immunology; Male; Mice; Mice, Inbred C57BL; Neovascularization, Physiologic - drug effects; P-Selectin - biosynthesis; P-Selectin - drug effects; P-Selectin - immunology; Phenotype; Platelet Endothelial Cell Adhesion Molecule-1 - biosynthesis; Platelet Endothelial Cell Adhesion Molecule-1 - drug effects; Platelet Endothelial Cell Adhesion Molecule-1 - immunology; Tumor Necrosis Factor-alpha - pharmacology; Vascular Cell Adhesion Molecule-1 - biosynthesis; Vascular Cell Adhesion Molecule-1 - drug effects; Vascular Cell Adhesion Molecule-1 - immunology
• ISSN: 1073-9688; 1073-9688
• DOI: 10.1038/sj.mn.7300113

Recent studies indicate that endothelial cells of newly formed blood vessels are activated and exhibit a distinct phenotype that may influence the responses of these microvessels to an inflammatory stimulus. The objective of this study was to compare the basal and cytokine-stimulated expression of endothelial cell adhesion molecules in neovascularized tissue to normal (nonproliferating) vascular beds. The expression of P- and E-selectin. VCAM-1, ICAM-1, ICAM-2, and PECAM-1 was measured, using the dual radiolabeled mAb technique, in subcutaneously implanted (for 10-15 days) polyurethane sponges, skin, heart, lung, and intestine of male C57BL/6 mlice (background). Basal values of PECAM-1 and ICAM-2 revealed a low vascular density in the implanted sponge matrices that is comparable to skin. When normalized for vascular surface area (PECAM-1 or ICAM-1 expression), the basal level of E- and P-selectin expression was highest in neovascularized sponge and skin. TNF-alpha elicited an increased expression of all endothelial CAMs, except PECAM-1 and ICAM-2, but the responses were blunted in sponge and skin, relative to other vascular beds. These findings indicate that endothelial cells in newly formed blood vessels exhibit a pattern of basal and cytokine-induced expression of certain adhesion glycoproteins that is similar to nonproliferating cutaneous vessels.