Diagnosis and Monitoring of Human Cytomegalovirus Diseases in Patients with Human Immunodeficiency Virus Infection by Use of a Real-Time PCR Assay

We used a real-time PCR assay to measure human cytomegalovirus (HCMV) DNA load in whole blood and plasma of 70 patients who were infected with human immunodeficiency virus type 1. Break points of 3.0 × 103 copies/mL in whole blood and 1.0 × 103 copies/mL in plasma were well-correlated with the exist...

Full description

Saved in:
Bibliographic Details
Published in:Clinical infectious diseases 2001-11, Vol.33 (10), p.1756-1761
Main Authors: Yoshida, Atsushi, Hitomi, Shigemi, Fukui, Takafumi, Endo, Hirohisa, Morisawa, Yuji, Kazuyama, Yukumasa, Osumi, Kazuoki, Oka, Shinichi, Kimura, Satoshi
Format: Article
Language:English
Subjects:
Adult
Aged
AIDS
AIDS-Related Opportunistic Infections - diagnosis
AIDS-Related Opportunistic Infections - virology
Biological and medical sciences
Blood
Blood plasma
Cytomegalovirus
Cytomegalovirus - genetics
Cytomegalovirus - isolation & purification
Cytomegalovirus infections
Cytomegalovirus Infections - diagnosis
Cytomegalovirus Infections - physiopathology
Cytomegalovirus Infections - virology
DNA, Viral - blood
HIV/AIDS
Human viral diseases
Humans
Infections
Infectious diseases
Medical sciences
Middle Aged
Polymerase chain reaction
Polymerase Chain Reaction - methods
Predictive Value of Tests
Retinitis
T lymphocytes
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Viral Load
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We used a real-time PCR assay to measure human cytomegalovirus (HCMV) DNA load in whole blood and plasma of 70 patients who were infected with human immunodeficiency virus type 1. Break points of 3.0 × 103 copies/mL in whole blood and 1.0 × 103 copies/mL in plasma were well-correlated with the existence of definite HCMV disease (sensitivity, 93% and 86%; specificity, 89% and 85%; positive predictive value, 70% and 63%; and negative predictive value, 98% and 95%, respectively). In patients with
ISSN:1058-4838
1537-6591
DOI:10.1086/323782