Preparation of Specific Antisera to Digoxin by Using Digoxin C-3' and C-3" Hemisuccinate-Bovine Serum Albumin Conjugates

The preparation and antigenic properties of digoxin C-3' and C-3" hemisuccinate-bovine serum albumin (BSA) conjugates are described. The antisera were prepared by immunizing rabbits with each of the digoxin-BSA conjugates, and properties of the antisera were characterized by RIA with 3H-la...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2000/08/01, Vol.23(8), pp.906-910
Main Authors: IKEDA, Yukari, FUJII, Youichi
Format: Article
Language:English
Subjects:
anti-digoxin antiserum
Biological and medical sciences
Cardiotonic agents
Cardiovascular system
Cross Reactions
digoxin
Digoxin - immunology
digoxin hemisuccinate
digoxin-BSA conjugate
Immune Sera
Medical sciences
Pharmacology. Drug treatments
Radioimmunoassay
RIA
Serum Albumin, Bovine - chemistry
Serum Albumin, Bovine - immunology
Succinates - chemistry
Succinates - immunology
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Description
Summary:The preparation and antigenic properties of digoxin C-3' and C-3" hemisuccinate-bovine serum albumin (BSA) conjugates are described. The antisera were prepared by immunizing rabbits with each of the digoxin-BSA conjugates, and properties of the antisera were characterized by RIA with 3H-labeled digoxin. The antidigoxin antiserum from immunization with digoxin 3'-hemisuccinate-BSA conjugate possessed high specificity for digoxin, exhibiting fairly low cross-reactions with dihydrodigoxin (2.1%), digoxigenin monodigitoxoside (0.9%), digoxigenin bisdigitoxoside (0.6%), and digoxigenin (0.1%).
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.23.906