Intracytoplasmic sperm injection of in vitro matured oocytes of domestic cats with frozen-thawed epididymal spermatozoa

The ability to mature and fertilize oocytes of endangered species may allow us to sustain genetic and global biodiversity. The first objective of this study was to compare the effect of two different culture media and two different incubation times on in vitro maturation (IVM) of domestic cat oocyte...

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Bibliographic Details
Published in:Theriogenology 2001-09, Vol.56 (5), p.955-967
Main Authors: Bogliolo, L, Leoni, G, Ledda, S, Naitana, S, Zedda, M, Carluccio, A, Pau, S
Format: Article
Language:English
Subjects:
Animals
Blastocyst - physiology
cat
Cats
Cells, Cultured
Cryopreservation
Culture Media
embryo
Epididymis - cytology
Female
ICSI
IVC
IVM
Male
Metaphase
Morula - physiology
oocyte
Oocytes - physiology
Sperm Injections, Intracytoplasmic - veterinary
Spermatozoa - physiology
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Summary:The ability to mature and fertilize oocytes of endangered species may allow us to sustain genetic and global biodiversity. The first objective of this study was to compare the effect of two different culture media and two different incubation times on in vitro maturation (IVM) of domestic cat oocytes. The second objective was to determine the developmental competence of in vitro matured cat oocytes after intracytoplasmic sperm injection (ICSI) with cat spermatozoa. Oocytes recovered from ovaries of ovariectomized cats were cultured either in TCM 199 medium or in synthetic oviductal fluid (SOF), both of which were supplemented with cysteamine, BSA, FSH, LH. Nuclear maturation was assessed after 24 h and 40 h of incubation. Results of IVM showed that the percentage of oocytes reaching MII after 24 h and 40 h of incubation were significantly higher (P
ISSN:0093-691X
1879-3231
DOI:10.1016/S0093-691X(01)00621-5