Growth hormone plus high protein diet promotes adaptation after massive bowel resection in aged rats

Objective. To determine whether GH improves adaptation following massive bowel resection in the aged rat fed on a high protein-content diet. Material and methods. Seventy-seven male Wistar rats aged 22±1 months underwent 80% bowel resection or laparotomy (sham-operation). They were randomly placed i...

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Bibliographic Details
Published in:Experimental gerontology 2001-11, Vol.36 (10), p.1727-1737
Main Authors: Fadrique, Begoña, Lopez, Juan de M., Bermudez, Raul, Gomez de Segura, Ignacio A., Vazquez, Isabel, De Miguel, Enrique
Format: Article
Language:English
Subjects:
Adaptation
Adaptation, Physiological
Aging - physiology
Animal
Animals
Body Weight
Cell Division - drug effects
Dietary Proteins - administration & dosage
Dietary Proteins - pharmacology
Elderly
Growth Hormone - pharmacology
Intestinal Mucosa - drug effects
Intestine
Intestines - drug effects
Intestines - pathology
Intestines - surgery
Nutrition
Postoperative Period
Rats
Rats, Wistar
Regulatory peptides
Surgery
Survival Analysis
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Summary:Objective. To determine whether GH improves adaptation following massive bowel resection in the aged rat fed on a high protein-content diet. Material and methods. Seventy-seven male Wistar rats aged 22±1 months underwent 80% bowel resection or laparotomy (sham-operation). They were randomly placed into one of eight groups, treated with either growth hormone (1 mg/kg/day) or saline, and fed a liquid diet containing either a high or a normal protein content. Intestinal tissue and blood samples were taken seven days after surgery and analysed to measure intestinal mucosal proliferation and mucosal height, as well as plasma levels of IGF-1 and somatostatin. Results. Resection of the small bowel in aged rats remarkably increased villous height and crypt proliferation. Growth hormone did not potentiate the increase in mucosal height and crypt proliferation observed after intestinal resection in aged rats fed a normal protein content diet, but did in those receiving a high-protein diet. Plasma levels of IGF-1 and somatostatin were not modified by surgery or treatment. Conclusion. Growth hormone may increase the adaptation of intestinal mucosa in aged rats undergoing massive intestinal resection, but requires an adequate nutritional support with increased amounts of high quality protein.
ISSN:0531-5565
1873-6815
DOI:10.1016/S0531-5565(01)00119-X