Annexin expressions are temporally and spatially regulated during rat hepatocyte differentiation

Annexin (Anx) 1, 2, 5, and 6 expressions were determined at the transcriptional and translational levels in the rat hepatocytes from gestational day 15 to postnatal day 17. Dramatic shifts were observed in Anx 1 and 2 levels, which peaked at day 1 and gestational day 20, respectively, and reached lo...

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Bibliographic Details
Published in:Developmental dynamics 2001-10, Vol.222 (2), p.206-217
Main Authors: Della Gaspera, Bruno, Braut‐Boucher, Françoise, Bomsel, Morgane, Chatelet, Françoise, Guguen‐Guillouzo, Christiane, Font, Jacqueline, Weinman, Jacqueline, Weinman, Serge
Format: Article
Language:English
Subjects:
Animals
Annexin A1 - genetics
Annexin A2 - genetics
Annexin A5 - genetics
Annexin A6 - genetics
annexins
Annexins - genetics
Cell Differentiation
Cells, Cultured
development
Dexamethasone - pharmacology
Epidermal Growth Factor - pharmacology
Female
Gene Expression Regulation, Developmental - drug effects
Gene Expression Regulation, Developmental - physiology
Glucocorticoids - pharmacology
Hepatocyte Growth Factor - pharmacology
hepatocytes
Hepatocytes - cytology
Liver - cytology
Liver - embryology
Male
Pregnancy
rat
Rats
Rats, Sprague-Dawley
RNA, Messenger - analysis
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Summary:Annexin (Anx) 1, 2, 5, and 6 expressions were determined at the transcriptional and translational levels in the rat hepatocytes from gestational day 15 to postnatal day 17. Dramatic shifts were observed in Anx 1 and 2 levels, which peaked at day 1 and gestational day 20, respectively, and reached low levels thereafter. However, Anx 5 and 6 rates were more constant. Prenatal administration of dexamethasone (dex) resulted in a decrease of Anx 1 mRNA levels, and a strong increase in Anx 2 mRNA contents. In adult hepatocytes cultured in the presence of EGF or HGF, Anx 1 and 2 expressions resumed. By immunohistochemistry, Anx 1 was detected only in the cytoplasm of hepatocytes of 1‐ to 3‐day‐old rats, Anx 2 and 6 both exhibited a redistribution from the cytoplasm toward the plasma membrane, and Anx 5 was present in the nucleus, cytoplasm, and plasma membrane. Thus, Anx 1, 2, 5, and 6 have individual modes of expression and localization in the differentiating hepatocytes, where they might play unique roles at well defined phases of liver ontogeny. © 2001 Wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/dvdy.1183