In vitro susceptibilities of Bartonella and Rickettsia spp. to fluoroquinolone antibiotics as determined by immunofluorescent antibody analysis of infected Vero cell monolayers

The in vitro susceptibilities of Bartonella and Rickettsia spp. to different concentrations of ciprofloxacin, levofloxacin, ofloxacin and sparfloxacin in Vero cell cultures, were determined by enumeration of immunofluorescent-stained bacilli. After incubation in a CO 2-enriched atmosphere, inocula w...

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Bibliographic Details
Published in:International journal of antimicrobial agents 2001-09, Vol.18 (3), p.217-222
Main Authors: Ives, Timothy J, Marston, Eric L, Regnery, Russell L, Butts, John D
Format: Article
Language:English
Subjects:
Animals
Anti-Infective Agents - pharmacology
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Bartonella
Bartonella - drug effects
Biological and medical sciences
Cercopithecus aethiops
Colony Count, Microbial
Fluorescent Antibody Technique
Fluoroquinolones
In vitro
Medical sciences
Microbial Sensitivity Tests
Pharmacology. Drug treatments
Rickettsia
Rickettsia - drug effects
Vero Cells - microbiology
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Summary:The in vitro susceptibilities of Bartonella and Rickettsia spp. to different concentrations of ciprofloxacin, levofloxacin, ofloxacin and sparfloxacin in Vero cell cultures, were determined by enumeration of immunofluorescent-stained bacilli. After incubation in a CO 2-enriched atmosphere, inocula were replaced and tested with media containing 12 different concentrations of each antibiotic in replicate for each species and the monolayers were re-incubated. Growth status was determined by evaluation of immunofluorescent staining bacilli. Effective inhibitory antibiotic dilution endpoints were determined by counting Bartonella- and Rickettsia-specific fluorescent foci across a range of antibiotic dilutions with an epi-fluorescent microscope, and were compared with an antibiotic-negative control. Based upon the use of C max:MIC and AUC:MIC data, levofloxacin exhibited activity against Bartonella elizabethae and B. quintana.
ISSN:0924-8579
1872-7913
DOI:10.1016/S0924-8579(01)00388-0