Delineation of the 6p22 amplification unit in urinary bladder carcinoma cell lines

Eight cell lines from transitional cell carcinoma of the urinary bladder were analyzed by comparative genomic hybridization. All tumor lines exhibited frequent chromosome gains (11.5/cell line) and losses (8.4/cell line). In six cell lines, gain of chromosome 5p was associated with gains of 6p and 2...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2000-08, Vol.60 (16), p.4526-4530
Main Authors: BRUCH, J, SCHULZ, W. A, HÄUSSLER, J, MELZNER, I, BRÜDERLEIN, S, MÖLLER, P, KEMMERLING, R, VOGEL, W, HAMEISTER, H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Carcinoma, Transitional Cell - genetics
chromosome 20
chromosome 5
chromosome 6
Chromosome Mapping
Chromosomes, Artificial, Yeast
Chromosomes, Human, Pair 20 - genetics
Chromosomes, Human, Pair 5 - genetics
Chromosomes, Human, Pair 6 - genetics
DNA, Neoplasm - genetics
Gene Amplification
High Mobility Group Proteins - genetics
Humans
In Situ Hybridization, Fluorescence
Medical sciences
Nephrology. Urinary tract diseases
Nucleic Acid Hybridization
Oncogenes
Polymerase Chain Reaction
PRL gene
Prolactin - genetics
SOX4 gene
SOXC Transcription Factors
Trans-Activators - genetics
Tumor Cells, Cultured
Tumors of the urinary system
Urinary Bladder Neoplasms - genetics
Urinary tract. Prostate gland
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Summary:Eight cell lines from transitional cell carcinoma of the urinary bladder were analyzed by comparative genomic hybridization. All tumor lines exhibited frequent chromosome gains (11.5/cell line) and losses (8.4/cell line). In six cell lines, gain of chromosome 5p was associated with gains of 6p and 20q. In five of these cell lines, amplification of parts of 6p was observed. Cytogenetic investigation combined with fluorescence in situ hybridization analysis revealed typical marker chromosomes with homogeneously staining regions (HSRs) containing material from 6p. By hybridizing individual yeast artificial chromosome probes from a chromosome 6p contig to these HSRs, a contig of three yeast artificial chromosomes common to all 6p HSRs was identified that spans less than 2 Mb. The genes SOX4 and PRL were shown to map to this region and to be coamplified in the cell lines. However, SOX4 was not overexpressed in any cell line and PRL was not expressed at all. Thus, the presumptive 6p oncogene remains to be conclusively identified.
ISSN:0008-5472
1538-7445