Effect of estrogen on the expression of Cry1 and Cry2 mRNAs in the suprachiasmatic nucleus of female rats

To determine whether estrogen has an effect on clock genes in the suprachiasmatic nucleus (SCN), we examined the effect of estrogen on the expression of Cry1 and Cry2 mRNAs in the SCN of female rats. Ovariectomized rats were injected with 20 μg 17β-estradiol at zeitgeber time (ZT) 6 and ZT 18 and ki...

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Bibliographic Details
Published in:Neuroscience research 2001-11, Vol.41 (3), p.251-255
Main Authors: Nakamura, Takahiro J, Shinohara, Kazuyuki, Funabashi, Toshiya, Kimura, Fukuko
Format: Article
Language:English
Subjects:
Animals
Biological Clocks - drug effects
Biological Clocks - genetics
Circadian Rhythm
Clock gene
Cryptochrome
Cryptochromes
Drosophila Proteins
Estradiol - pharmacology
Estrogen
Eye Proteins
Female
Flavoproteins - genetics
Northern blot
Ovariectomy
Photoreceptor Cells, Invertebrate
Rats
Rats, Wistar
Receptors, G-Protein-Coupled
RNA, Messenger - metabolism
Suprachiasmatic nucleus
Suprachiasmatic Nucleus - metabolism
Time Factors
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Summary:To determine whether estrogen has an effect on clock genes in the suprachiasmatic nucleus (SCN), we examined the effect of estrogen on the expression of Cry1 and Cry2 mRNAs in the SCN of female rats. Ovariectomized rats were injected with 20 μg 17β-estradiol at zeitgeber time (ZT) 6 and ZT 18 and killed 24 h after the treatment. Northern blot revealed that the expression of Cry2 mRNA was significantly increased in the SCN of estrogen-treated rats at both time points. But estrogen did not affect Cry1 mRNA levels in the SCN at any ZT. These results suggested that Cry1 and Cry2 mRNAs in the SCN were differently regulated by estrogen.
ISSN:0168-0102
1872-8111
DOI:10.1016/S0168-0102(01)00285-1