Acute and long-term effects of booster immunisation on frequencies of antigen-specific memory B-lymphocytes

In search for a parameter that is predictive of long-term immunity, we analysed the influence of booster immunisations on frequencies of circulating memory B-lymphocytes. Specific IgG-secreting B-cells were determined by ELISPOTassay in 13 healthy adults, using diphtheria and tetanus toxoid as model...

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Bibliographic Details
Published in:Vaccine 2001-11, Vol.20 (3), p.498-504
Main Authors: Nanan, Ralph, Heinrich, Daniela, Frosch, Mathias, Kreth, Hans Wolfgang
Format: Article
Language:English
Subjects:
Adult
Antigens - immunology
B-Lymphocytes - immunology
Biological and medical sciences
Booster immunisation
Epidemiology. Vaccinations
Female
Frequency analysis
Fundamental and applied biological sciences. Psychology
Fundamental immunology
General aspects
Humans
Immunization, Secondary
Immunobiology
Immunologic Memory
Infectious diseases
Lymphocyte Count
Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation
Male
Medical sciences
Memory B-lymphocytes
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Summary:In search for a parameter that is predictive of long-term immunity, we analysed the influence of booster immunisations on frequencies of circulating memory B-lymphocytes. Specific IgG-secreting B-cells were determined by ELISPOTassay in 13 healthy adults, using diphtheria and tetanus toxoid as model antigens. Our results show that memory B-cells accumulate with every immunisation dose and remain elevated over several years. In addition, secondary B-cell responses were studied during the first 90 days after diphtheria re-immunisation. A significant indirect correlation was found between the number of previous boosters and the magnitude of specific B-cell expansion. In contrast, effects of booster immunisations did not correlate likewise with antigen-specific serology. Hence, this study illustrates that frequencies of antigen-specific B-lymphocytes can be used as an indirect measure for immunological memory. This parameter could be helpful to find scientifically based immunisation strategies for currently available and novel vaccines.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(01)00328-0