Acute and long-term effects of booster immunisation on frequencies of antigen-specific memory B-lymphocytes

• Published in: Vaccine 2001-11, Vol.20 (3), p.498-504
• Main Authors: Nanan, Ralph, Heinrich, Daniela, Frosch, Mathias, Kreth, Hans Wolfgang
• Format: Article
• Language: English
• Subjects: Adult; Antigens - immunology; B-Lymphocytes - immunology; Biological and medical sciences; Booster immunisation; Epidemiology. Vaccinations; Female; Frequency analysis; Fundamental and applied biological sciences. Psychology; Fundamental immunology; General aspects; Humans; Immunization, Secondary; Immunobiology; Immunologic Memory; Infectious diseases; Lymphocyte Count; Lymphoid cells: ontogeny, maturation, markers, receptors, circulation and recirculation; Male; Medical sciences; Memory B-lymphocytes
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(01)00328-0

In search for a parameter that is predictive of long-term immunity, we analysed the influence of booster immunisations on frequencies of circulating memory B-lymphocytes. Specific IgG-secreting B-cells were determined by ELISPOTassay in 13 healthy adults, using diphtheria and tetanus toxoid as model antigens. Our results show that memory B-cells accumulate with every immunisation dose and remain elevated over several years. In addition, secondary B-cell responses were studied during the first 90 days after diphtheria re-immunisation. A significant indirect correlation was found between the number of previous boosters and the magnitude of specific B-cell expansion. In contrast, effects of booster immunisations did not correlate likewise with antigen-specific serology. Hence, this study illustrates that frequencies of antigen-specific B-lymphocytes can be used as an indirect measure for immunological memory. This parameter could be helpful to find scientifically based immunisation strategies for currently available and novel vaccines.