Differential expression of cytoplasmic carbonic anhydrases, CA I and II, and membrane-associated isozymes, CA IX and XII, in normal mucosa of large intestine and in colorectal tumors

This study compares the localization of carbonic anhydrase isozymes (CA) I and II and that of IX and XII in normal large intestine and in colorectal tumors. Immunohistochemical studies were performed on 69 colorectal lesions. While the normal mucosa of the large intestine showed high expression for...

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Bibliographic Details
Published in:Digestive diseases and sciences 2001-10, Vol.46 (10), p.2179-2186
Main Authors: KIVELÄ, Antti J, SAARNIO, Juha, RAJANIEMI, Hannu, KARTTUNEN, Tuomo J, KIVELÄ, Jyrki, PARKKILA, Anna-Kaisa, PASTOREKOVA, Silvia, PASTOREK, Jaromir, WAHEED, Abdul, SLY, William S, PARKKILA, Seppo
Format: Article
Language:English
Subjects:
Adenocarcinoma - metabolism
Adenomatous Polyps - metabolism
Antigens, Neoplasm
Biological and medical sciences
Carbonic Anhydrase I - metabolism
Carbonic Anhydrase II - metabolism
Carbonic Anhydrase IX
Carbonic Anhydrases - metabolism
Colorectal Neoplasms - metabolism
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Immunohistochemistry
Intestinal Mucosa - metabolism
Medical sciences
Neoplasm Proteins - metabolism
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
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Summary:This study compares the localization of carbonic anhydrase isozymes (CA) I and II and that of IX and XII in normal large intestine and in colorectal tumors. Immunohistochemical studies were performed on 69 colorectal lesions. While the normal mucosa of the large intestine showed high expression for CA I and II, the intensity of the immunostaining for both isozymes decreased in benign lesions and was very weak in malignant tumors. The reciprocal pattern of expression observed for these cytoplasmic isozymes and transmembrane CA IX and XII in intestinal tissue specimens supports the suggestion that CA IX and XII may be functionally involved in tumor progression to malignancy and/or in invasion. By contrast, while CA I and II are prominent in normal colorectal mucosa, where they play a role in regulation of pH homeostasis and water and ion transport, loss of expression of these cytoplasmic isozymes consistently accompanies progression to malignant transformation.
ISSN:0163-2116
1573-2568
DOI:10.1023/A:1011910931210