Identification and characterization of two novel calpain large subunit genes

Calpains are a family of related proteins, some of which have been shown to function as calcium-dependent cysteine proteases. CAPN1 and CAPN2, the most well characterized calpains, consist of a large (80 kDa) and a small (30 kDa) subunit. In mammals, 11 different paralogous genes encoding calpain la...

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Bibliographic Details
Published in:Gene 2001-08, Vol.274 (1), p.245-252
Main Authors: Dear, T.Neil, Boehm, Thomas
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Blotting, Northern
Calcium
Calpain - genetics
CAPN13 gene
CAPN14 gene
chromosome 1
chromosome 11
chromosome 15
chromosome 19
chromosome 2
chromosome 3
chromosome 6
Chromosomes, Human, Pair 2 - genetics
Cysteine protease
Expression
Gene Expression
Gene Order
Gene structure
Humans
Male
Molecular Sequence Data
Multigene Family - genetics
Phylogeny
Protein Subunits
Radiation Hybrid Mapping
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Tissue Distribution
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Summary:Calpains are a family of related proteins, some of which have been shown to function as calcium-dependent cysteine proteases. CAPN1 and CAPN2, the most well characterized calpains, consist of a large (80 kDa) and a small (30 kDa) subunit. In mammals, 11 different paralogous genes encoding calpain large subunits have been identified. We report the identification of two further genes, CAPN13 and CAPN14, potentially encoding calpain large subunits. Radiation hybrid mapping localized both genes within a region mapped to 2p21-2p22. The CAPN13 mRNA exhibits a restricted tissue distribution with low levels of expression detected only in human testis and lung while CAPN14 mRNA could not be detected in any of the 76 tissues examined. Examination of the human genome sequence in the public and private consortia databases did not detect any further members of this gene family. Thus, there would seem to be 13 large subunit calpain genes in the human genome. Phylogenetic analysis reveals that the putative calpain large subunit proteins can be divided into three major groups. The 13 human large subunit genes and the single small subunit gene are located in eight syntenic groups on chromosomes 1, 2, 3, 6, 11, 15, 19 and X.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(01)00599-6