Estradiol Increases IRS-1 Gene Expression and Insulin Signaling in Breast Cancer Cells

This study demonstrates how the potentiating effects of E2 on insulin signaling in endoplasmic reticulum-positive breast cancer cells are consequent to an enhanced IRS-1 expression. It induces an increase of both PI-3K/AKT and ERK1/2 activities. A direct action of E2 in the regulating mouse IRS-1 ge...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2001-11, Vol.288 (3), p.685-689
Main Authors: Mauro, Loredana, Salerno, Michele, Panno, Maria Luisa, Bellizzi, Dina, Sisci, Diego, Miglietta, Antonella, Surmacz, Eva, Andò, Sebastiano
Format: Article
Language:English
Subjects:
Analysis of Variance
breast cancer
Breast Neoplasms
estradiol
Estradiol - pharmacology
Gene Expression Regulation - drug effects
Humans
Insulin - physiology
Insulin Receptor Substrate Proteins
insulin signaling
IRS-1 gene expression
IRS-1 protein
Phosphoproteins - genetics
Phosphoproteins - metabolism
Phosphorylation
Promoter Regions, Genetic - drug effects
Promoter Regions, Genetic - genetics
RNA, Messenger - drug effects
RNA, Messenger - metabolism
Signal Transduction - drug effects
Signal Transduction - physiology
Tumor Cells, Cultured
Tyrosine - metabolism
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Summary:This study demonstrates how the potentiating effects of E2 on insulin signaling in endoplasmic reticulum-positive breast cancer cells are consequent to an enhanced IRS-1 expression. It induces an increase of both PI-3K/AKT and ERK1/2 activities. A direct action of E2 in the regulating mouse IRS-1 gene is also investigated in both Chinese hamster ovary and MCF-7 cells that are transfected with mouse IRS-1 regulatory sequences. The authors have reported, for the first time, how E2 induction of IRS-1 mRNA was correlated with a direct positive regulatory role of E2 on the IRS-1 promoter. This effect seems to be not strictly related to the cell type.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.2001.5815