Nm23-H1 gene as a molecular switch between the free-floating and adherent states of gastric cancer cells

The contribution of the nm23-H1 gene to metastasis in malignant tumors, including gastric cancer, is controversial. In this study, we compared nm23-H1 levels in two cell subtypes with different morphologies (floating and adherent states), but that were derived from the same gastric cancer cell line,...

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Bibliographic Details
Published in:Cancer letters 2001-12, Vol.174 (1), p.65-71
Main Authors: Iizuka, Norio, Tangoku, Akira, Hazama, Shoichi, Yoshino, Shigefumi, Mori, Naohide, Oka, Masaaki
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Adhesion - genetics
Cell Cycle
Dissemination
Flow Cytometry
Gastric cancer
gastric carcinoma
Humans
Medical sciences
Metastasis
Monomeric GTP-Binding Proteins - genetics
Monomeric GTP-Binding Proteins - metabolism
Neoplasm Metastasis - genetics
Neoplasm Metastasis - pathology
NM23
NM23 Nucleoside Diphosphate Kinases
nm23-H1 gene
Nucleoside diphosphate kinase
Real-time quantitative RT-PCR
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Stomach Neoplasms - genetics
Stomach Neoplasms - pathology
Transcription Factors - genetics
Transcription Factors - metabolism
Tumor cell
Tumor Cells, Cultured
Tumors
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Summary:The contribution of the nm23-H1 gene to metastasis in malignant tumors, including gastric cancer, is controversial. In this study, we compared nm23-H1 levels in two cell subtypes with different morphologies (floating and adherent states), but that were derived from the same gastric cancer cell line, KATO-III. A real-time quantitative reverse transcription-polymerase chain reaction showed that the number of nm23-H1 mRNA molecules in floating cells was significantly higher than that in adherent cells ( P
ISSN:0304-3835
1872-7980
DOI:10.1016/S0304-3835(01)00684-X