Molecular cloning and characterization of a metal responsive Aedes aegypti intestinal mucin cDNA

We have isolated a cDNA from Aedes aegypti that is transcribed in the larval midgut in response to metal exposure, and in the adult female midgut in response to iron or cadmium exposure, or a blood meal. The cDNA encodes a protein, designated Aedes aegypti intestinal mucin 1 (AEIMUC1), which has sim...

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Bibliographic Details
Published in:Insect molecular biology 2000-08, Vol.9 (4), p.419-426
Main Authors: Rayms-Keller, A., McGaw, M., Oray, C., Carlson, J. O., Beaty, B. J.
Format: Article
Language:English
Subjects:
Aedes - genetics
Aedes aegypti
AEIMUC1 gene
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Culicidae
Digestive System
DNA, Complementary
Female
Freshwater
Humans
intestinal mucin 1
Metals
Molecular Sequence Data
mucin
Mucins - genetics
peritrophin
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Summary:We have isolated a cDNA from Aedes aegypti that is transcribed in the larval midgut in response to metal exposure, and in the adult female midgut in response to iron or cadmium exposure, or a blood meal. The cDNA encodes a protein, designated Aedes aegypti intestinal mucin 1 (AEIMUC1), which has similarities with invertebrate intestinal mucins and peritrophins, and vertebrate mucins. Proline, serine and threonine comprise 30% of the amino acid composition of AEIMUC1, a characteristic of mucins. AEIMUC1 contains three cysteine‐rich domains, two of which flank a proline/serine/threonine‐rich domain, a feature shared by many mucin genes. This is the first report on the isolation of a metal‐responsive gene from an aquatic insect.
ISSN:0962-1075
1365-2583
DOI:10.1046/j.1365-2583.2000.00202.x