Molecular cloning and characterization of a metal responsive Aedes aegypti intestinal mucin cDNA

We have isolated a cDNA from Aedes aegypti that is transcribed in the larval midgut in response to metal exposure, and in the adult female midgut in response to iron or cadmium exposure, or a blood meal. The cDNA encodes a protein, designated Aedes aegypti intestinal mucin 1 (AEIMUC1), which has sim...

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Published in:Insect molecular biology 2000-08, Vol.9 (4), p.419-426
Main Authors: Rayms-Keller, A., McGaw, M., Oray, C., Carlson, J. O., Beaty, B. J.
Format: Article
Language:English
Subjects:
Aedes - genetics
Aedes aegypti
AEIMUC1 gene
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Culicidae
Digestive System
DNA, Complementary
Female
Freshwater
Humans
intestinal mucin 1
Metals
Molecular Sequence Data
mucin
Mucins - genetics
peritrophin
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cites cdi_FETCH-LOGICAL-c4332-9a0448d91679265f09679b8f47b5bb6f9441c2679705598bc63bf988df325bba3
container_end_page 426
container_issue 4
container_start_page 419
container_title Insect molecular biology
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creator Rayms-Keller, A.
McGaw, M.
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Carlson, J. O.
Beaty, B. J.
description We have isolated a cDNA from Aedes aegypti that is transcribed in the larval midgut in response to metal exposure, and in the adult female midgut in response to iron or cadmium exposure, or a blood meal. The cDNA encodes a protein, designated Aedes aegypti intestinal mucin 1 (AEIMUC1), which has similarities with invertebrate intestinal mucins and peritrophins, and vertebrate mucins. Proline, serine and threonine comprise 30% of the amino acid composition of AEIMUC1, a characteristic of mucins. AEIMUC1 contains three cysteine‐rich domains, two of which flank a proline/serine/threonine‐rich domain, a feature shared by many mucin genes. This is the first report on the isolation of a metal‐responsive gene from an aquatic insect.
doi_str_mv 10.1046/j.1365-2583.2000.00202.x
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subjects Aedes - genetics
Aedes aegypti
AEIMUC1 gene
Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Culicidae
Digestive System
DNA, Complementary
Female
Freshwater
Humans
intestinal mucin 1
Metals
Molecular Sequence Data
mucin
Mucins - genetics
peritrophin
title Molecular cloning and characterization of a metal responsive Aedes aegypti intestinal mucin cDNA
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